Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Kobe2602 是 Ras-Raf 相互作用抑制剂,具有抗癌化疗活性。它抑制 H-Ras·GTP 与 c-Raf-1 RBD 结合的Ki 值为 149 μM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
5 mg | ¥ 177 | 现货 | ||
10 mg | ¥ 321 | 现货 | ||
25 mg | ¥ 597 | 现货 | ||
50 mg | ¥ 882 | 现货 | ||
100 mg | ¥ 1,415 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 192 | 现货 |
产品描述 | Kobe 2602 is an inhibitor of Ras that exhibits anticancer chemotherapeutic activities. |
靶点活性 | Ras:149 μM(Ki) |
激酶实验 | Migration assays: Assays are conducted in wells of a 24-well, 8 μm pore size Transwell plate. A 6.5-mm insert membrane is coated with 0.1 mL COL-I (300 μg/mL in MEGM) and then air dried for 16 hours. The collagen coating is rehydrated for 1 hour in 0.2 mL MEGM. The inner chamber contains MEGM-10% FBS as a chemoattractant. The compounds (10–100 μmol/L) or DMSO (0.1%–1%) are added to both inner and outer chambers. Before plating in the outer chamber, cells (5×104) are coincubated for 20 minutes with the compounds or DMSO in MEGM. Cells are allowed to migrate for 16 to 18 hours. The cells remaining on the top surface of the membrane are removed with a cotton swab. The cells on the bottom surface of the membrane are fixed and stained (0.2% crystal violet). The incorporated dye is extracted with 1% SDS and the A570 is measured. |
分子量 | 419.31 |
分子式 | C14H9F4N5O4S |
CAS No. | 454453-49-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 50 mg/mL (119.24 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.3849 mL | 11.9244 mL | 23.8487 mL | 59.6218 mL |
5 mM | 0.477 mL | 2.3849 mL | 4.7697 mL | 11.9244 mL | |
10 mM | 0.2385 mL | 1.1924 mL | 2.3849 mL | 5.9622 mL | |
20 mM | 0.1192 mL | 0.5962 mL | 1.1924 mL | 2.9811 mL | |
50 mM | 0.0477 mL | 0.2385 mL | 0.477 mL | 1.1924 mL | |
100 mM | 0.0238 mL | 0.1192 mL | 0.2385 mL | 0.5962 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Kobe2602 454453-49-7 GPCR/G Protein MAPK Raf Ras H-Ras Inhibitor antiproliferative Kobe-2602 carcinoma Kobe 2602 inhibit inhibitor