Powder: -20°C for 3 years | In solvent: -80°C for 1 year
KKL-35 是一种具有广谱抗生素活性的反式翻译抑制剂,IC50值为 0.9 µM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 437 | 现货 | ||
2 mg | ¥ 636 | 现货 | ||
5 mg | ¥ 1,050 | 现货 | ||
10 mg | ¥ 1,730 | 现货 | ||
25 mg | ¥ 3,490 | 现货 | ||
50 mg | ¥ 5,390 | 现货 | ||
100 mg | ¥ 7,890 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 1,380 | 现货 |
产品描述 | KKL-35 is an inhibitor of trans-translation (IC50: 0.9 μM) with broad-spectrum antibiotic activity. |
靶点活性 | trans-translation tagging reaction:0.9 µM |
体外活性 | KKL-35 inhibited tagging of DHFR-ns. Dose-response experiments showed that KKL-35 inhibited tagging with an IC50 = 0.9 μM. KKL-35 prevented the growth of WT S. flexneri with a minimum inhibitory concentration (MIC) of 6 μM, and the addition of KKL-35 to a growing culture of S. flexneri stops growth [1]. Consistent with the essential nature of trans-translation in L. pneumophila, KKL-35 inhibited the growth of all tested strains at submicromolar concentrations. KKL-35 remained equally active against L. pneumophila mutants that have evolved resistance to macrolides. KKL-35 inhibited the multiplication of L. pneumophila in human macrophages at several stages of infection [2]. |
细胞实验 | Overnight cultures of S. flexneri 2a 2457T, B. anthracis, M. smegmatis, JW5503, and NSR253 were grown in lysogeny broth (LB). Cultures were diluted to a final inoculum of ~5 × 10^5 cfu/mL in 96-well microtiter plates, compounds were added at appropriate concentrations, and growth was observed after 24-h incubation at 37 °C. The MIC was determined by the concentration of the compound in the last well that showed no bacterial growth. For MBC assays, 10 μL from wells containing the MIC, 2× MIC, and 4× MIC of each compound was diluted 10-fold, spotted on LB plates, and grown overnight at 37 °C. An inhibitor was scored as bactericidal if it resulted in 1,000-fold reduction in colony-forming units per milliliter from the original inoculum [1]. |
分子量 | 317.7 |
分子式 | C15H9ClFN3O2 |
CAS No. | 865285-29-6 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 8.33 mg/mL (26.23 mM), Sonification is recommended
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.1476 mL | 15.7381 mL | 31.4762 mL | 78.6906 mL |
5 mM | 0.6295 mL | 3.1476 mL | 6.2952 mL | 15.7381 mL | |
10 mM | 0.3148 mL | 1.5738 mL | 3.1476 mL | 7.8691 mL | |
20 mM | 0.1574 mL | 0.7869 mL | 1.5738 mL | 3.9345 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
KKL-35 865285-29-6 Microbiology/Virology Antibacterial Bacterial Inhibitor KKL 35 inhibit KKL35 inhibitor