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KKL-35

KKL-35

产品编号 T5331   CAS 865285-29-6

KKL-35 是一种具有广谱抗生素活性的反式翻译抑制剂,IC50值为 0.9 µM。

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KKL-35 Chemical Structure
KKL-35, CAS 865285-29-6
规格 价格/CNY 货期 数量
1 mg ¥ 437 现货
2 mg ¥ 636 现货
5 mg ¥ 1,050 现货
10 mg ¥ 1,730 现货
25 mg ¥ 3,490 现货
50 mg ¥ 5,390 现货
100 mg ¥ 7,890 现货
1 mL * 10 mM (in DMSO) ¥ 1,380 现货
产品目录号及名称: KKL-35 (T5331)
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生物活性
化学信息
存储 & 溶解度
参考文献
产品描述 KKL-35 is an inhibitor of trans-translation (IC50: 0.9 μM) with broad-spectrum antibiotic activity.
靶点活性 trans-translation tagging reaction:0.9 µM
体外活性 KKL-35 inhibited tagging of DHFR-ns. Dose-response experiments showed that KKL-35 inhibited tagging with an IC50 = 0.9 μM. KKL-35 prevented the growth of WT S. flexneri with a minimum inhibitory concentration (MIC) of 6 μM, and the addition of KKL-35 to a growing culture of S. flexneri stops growth [1]. Consistent with the essential nature of trans-translation in L. pneumophila, KKL-35 inhibited the growth of all tested strains at submicromolar concentrations. KKL-35 remained equally active against L. pneumophila mutants that have evolved resistance to macrolides. KKL-35 inhibited the multiplication of L. pneumophila in human macrophages at several stages of infection [2].
细胞实验 Overnight cultures of S. flexneri 2a 2457T, B. anthracis, M. smegmatis, JW5503, and NSR253 were grown in lysogeny broth (LB). Cultures were diluted to a final inoculum of ~5 × 10^5 cfu/mL in 96-well microtiter plates, compounds were added at appropriate concentrations, and growth was observed after 24-h incubation at 37 °C. The MIC was determined by the concentration of the compound in the last well that showed no bacterial growth. For MBC assays, 10 μL from wells containing the MIC, 2× MIC, and 4× MIC of each compound was diluted 10-fold, spotted on LB plates, and grown overnight at 37 °C. An inhibitor was scored as bactericidal if it resulted in 1,000-fold reduction in colony-forming units per milliliter from the original inoculum [1].
分子量 317.7
分子式 C15H9ClFN3O2
CAS No. 865285-29-6

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

DMSO: 8.33 mg/mL (26.23 mM), Sonification is recommended

溶液配制表

可选溶剂 浓度 体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.1476 mL 15.7381 mL 31.4762 mL 78.6906 mL
5 mM 0.6295 mL 3.1476 mL 6.2952 mL 15.7381 mL
10 mM 0.3148 mL 1.5738 mL 3.1476 mL 7.8691 mL
20 mM 0.1574 mL 0.7869 mL 1.5738 mL 3.9345 mL

计算器

摩尔浓度计算器
稀释计算器
配液计算器
分子量计算器
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输入分子式,点击计算,可计算出产品的分子量。

参考文献

1. 1. Ramadoss NS, et al. Small molecule inhibitors of trans-translation have broad-spectrum antibiotic activity. Proc Natl Acad Sci U S A. 2013 Jun 18;110(25):10282-7. 2. Brunel R, et al. KKL-35 Exhibits Potent Antibiotic Activity against Legionella Species Independently of trans-Translation Inhibition. Antimicrob Agents Chemother. 2018 Jan 25;62(2). pii: e01459-17.
Novobiocin Sodium Grosvenorine Closthioamide Allyl Methyl Sulfide Phthalylsulfacetamide Erythromycin ethylsuccinate Cefdinir Moxifloxacin

相关化合物库

该产品包含在如下化合物库中:
抑制剂库 NO PAINS 化合物库 抗生素库 抗菌活性库 抗感染化合物库 肝脏毒性化合物库 已知活性化合物库 免疫/炎症分子化合物库 经典已知活性库

剂量换算

对于不同动物的给药剂量换算,您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。

体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。

第一步:请输入动物实验的基本信息
剂量
mg/kg
每只动物体重
g
给药体积
μL
动物数量
第二步:请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
% DMSO
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% Tween 80
% ddH2O
计算 重置

技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

KKL-35 865285-29-6 Microbiology/Virology Antibacterial Bacterial Inhibitor KKL 35 inhibit KKL35 inhibitor

 

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