Powder: -20°C for 3 years | In solvent: -80°C for 1 year
K777 是有效的,具有口服活性的,不可逆的半胱氨酸蛋白酶 (cysteine protease) 抑制剂。K777是有效的 CYP3A4 抑制剂(IC50= 60 nM)。K777也是选择性的 CCR4 拮抗剂,显示出强的趋化性抑制作用。K777 对 Cruzain(克鲁斯锥虫的主要半胱氨酸蛋白酶)和组织蛋白酶 B 和 L 具有不可逆的抑制作用。K777 通过靶向组织蛋白酶介导的细胞进入,显示出广谱的抗病毒作用。K777 抑制 EBOV 和 SARS-CoV 病毒的进入,IC50 值分别为 0.87 nM 和 0.68 nM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 638 | 6-8周 | ||
5 mg | ¥ 1,390 | 6-8周 | ||
10 mg | ¥ 2,160 | 6-8周 | ||
25 mg | ¥ 3,820 | 6-8周 | ||
50 mg | ¥ 5,320 | 6-8周 | ||
100 mg | ¥ 7,320 | 6-8周 | ||
500 mg | ¥ 14,600 | 6-8周 | ||
1 mL * 10 mM (in DMSO) | ¥ 1,780 | 6-8周 |
产品描述 | K777 is a potent, orally active and irreversible inhibitor of cysteine protease. K777 is a potent CYP3A4 inhibitor (IC50= 60 nM). K777 is also a selective CCR4 antagonist featuring the potent chemotaxis inhibition. K777 irreversibly inhibits Cruzain, which is the major cysteine protease of Trypansoma cruzi, and cathepsins B and L. K777 targets cathepsin-mediated cell entry and wxibits a broad-spectrum antiviral activity. K777 inhibits EBOV and SARS-CoV pseudovirus entry with IC50 values of 0.87 nM and 0.68 nM, respectively. |
靶点活性 | CYP3A4:60 nM (IC50) |
体外活性 | K777 is a broad-spectrum antiviral and inhibits SARS-CoV, HCoV-229E, NL63, MERS-CoV, EBOV, SUDV, TAFV, RESTV, BEBOV, MARV and Nipah pseudovirus entry with IC50 values of 0.68 nM, 1.48 nM, 6.78 nM, 46.12 nM, 0.87 nM, 1.14 nM, 2.26 nM, 3.37 nM, 5.91 nM, 1.9 nM and 0.42 nM, respectively. K777 alone demonstrates up to 70% inhibition of 229E-S-mediated transduction in TMPRSS2 expressing cells. Simultaneous treatment with Camostat and K777 increases inhibition to ~ 90%[1]. K777 inhibits both CCL17 binding and CCL17-induced chemotaxis in Hut78 cells with IC50s of 57 and 8.9 nM, respectively. The K777-mediated inhibition of chemotaxis is potent even in the presence of a 10-fold higher concentration of CCL17. K777 induces CCR4 internalization with a 50% reduction of cell surface CCR4[3]. |
体内活性 | In C57BL/6 IFN-γR-KO mice, K777 ( 35-105 mg/kg; p.o.) rescues mice from otherwise lethal infections[4]. |
别名 | APC-3316 |
分子量 | 574.73 |
分子式 | C32H38N4O4S |
CAS No. | 233277-99-1 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 90 mg/mL (156.6 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 1.7399 mL | 8.6997 mL | 17.3995 mL | 43.4987 mL |
5 mM | 0.348 mL | 1.7399 mL | 3.4799 mL | 8.6997 mL | |
10 mM | 0.174 mL | 0.87 mL | 1.7399 mL | 4.3499 mL | |
20 mM | 0.087 mL | 0.435 mL | 0.87 mL | 2.1749 mL | |
50 mM | 0.0348 mL | 0.174 mL | 0.348 mL | 0.87 mL | |
100 mM | 0.0174 mL | 0.087 mL | 0.174 mL | 0.435 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
K777 233277-99-1 Immunology/Inflammation Metabolism Microbiology/Virology Proteases/Proteasome P450 Cysteine Protease CCR APC 3316 K-777 APC-3316 K 777 APC3316 Inhibitor inhibitor inhibit