Powder: -20°C for 3 years | In solvent: -80°C for 1 year
JW 55 (JW55) 是一种有效且选择性的 β-catenin 信号通路抑制剂,通过抑制 tankyrase 1 和 tankyrase 2 (TNKS1/2) 的 PARP 结构域起作用。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
5 mg | ¥ 198 | 现货 | ||
10 mg | ¥ 347 | 现货 | ||
25 mg | ¥ 690 | 现货 | ||
50 mg | ¥ 1,120 | 现货 | ||
100 mg | ¥ 1,830 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 228 | 现货 |
产品描述 | JW 55 (JW55) is an effective and selective β-catenin signaling pathway inhibitor, works by inhibition of the PARP domain of tankyrase 1 and tankyrase 2 (TNKS1/2). |
靶点活性 | TNKS1 (auto-PARsylation):1.9 μM, TNKS2 (auto-PARsylation):830 nM |
体外活性 | JW 55 (JW55) is a potent and selective inhibitor of the canonical Wnt pathway. Wnt3a-induced HEK293 cells containing a transiently transfected ST-Luc (SuperTop-luciferase) reporter show inhibition by JW55 with an IC50 value of 470 nM. JW55 is effective in the range of 1 to 5 μM in SW480 cells and 0.01 to 5 μM in HCT-15 cells. JW55 is effective in the range of 1 to 5 μM in SW480 cells and 0.01 to 5 μM in HCT-15 cells[1]. |
体内活性 | JW 55 (100 mg/kg, orally) reduces tumor development in conditional Apc knockout mice. JW55 reduces XWnt8-induced axis duplication inXenopus embryos and Tamoxifen-induced polyposis formation in conditional APC mutant mice[1]. |
细胞实验 | JW 55 (JW55) is prepared in DMSO and stored, and then diluted with appropriate medium[1]. A total of 1,000 SW480 or RKO cells are seeded in 96-well plates. The day after, the cell culture medium is exchanged to solutions that contained 0.1% DMSO or 10 μM JW55 for RKO cells and 0.1% DMSO or 10, 5, or 1 μM JW55 for SW480 cells. All samples consist of a minimum of 6 replicates. The plate is incubated in an IncuCyte inside a cell culture incubator. Images are captured every second hour to monitor proliferation[1]. |
别名 | JW55 |
分子量 | 434.48 |
分子式 | C25H26N2O5 |
CAS No. | 664993-53-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: 100 mM
DMSO: 100 mM
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
Ethanol / DMSO | 1 mM | 2.3016 mL | 11.508 mL | 23.016 mL | 57.54 mL |
5 mM | 0.4603 mL | 2.3016 mL | 4.6032 mL | 11.508 mL | |
10 mM | 0.2302 mL | 1.1508 mL | 2.3016 mL | 5.754 mL | |
20 mM | 0.1151 mL | 0.5754 mL | 1.1508 mL | 2.877 mL | |
50 mM | 0.046 mL | 0.2302 mL | 0.4603 mL | 1.1508 mL | |
100 mM | 0.023 mL | 0.1151 mL | 0.2302 mL | 0.5754 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
JW 55 664993-53-7 Chromatin/Epigenetic Cytoskeletal Signaling DNA Damage/DNA Repair Stem Cells PARP Wnt/beta-catenin JW-55 poly ADP ribose polymerase Inhibitor inhibit JW55 inhibitor