store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
JSH-23 是NF-κB 抑制剂,作用于脂多糖刺激的巨噬细胞 RAW 264.7,抑制 NF-κB 转录活性(IC50=7.1 μM),抑制 NF-κB p65 的核易位而不影响 IκBα 降解。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 373 | 现货 | ||
2 mg | ¥ 542 | 现货 | ||
5 mg | ¥ 898 | 现货 | ||
10 mg | ¥ 1,220 | 现货 | ||
25 mg | ¥ 1,980 | 现货 | ||
50 mg | ¥ 2,970 | 现货 | ||
100 mg | ¥ 4,420 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 965 | 现货 |
产品描述 | JSH-23, an NF-κB inhibitor(IC50= 7.1 μM), suppresses the transcriptional activity. |
靶点活性 | NF-κB:7.1 μM |
体外活性 | 在糖尿病大鼠体内,JSH-23(3 mg/kg)能够减少神经炎症,并提高抗氧化防御明显逆转神经传导,最终改善神经血流量不足. |
体内活性 | 在RAW 264.7细胞中,JSH-23<100 μM时没有表现出明显的毒性作用,同时能够抑制脂多糖诱导的凋亡染色质浓缩。在老鼠小脑原代细胞中,JSH-23抑制脂多糖诱导的NF-κB p65核转运,而不影响IκBα降解。 |
激酶实验 | Measurement of NF-κB transcriptional activity: Macrophages RAW 264.7 transfected stably with reporter plasmid of pNF-κB-SEAP-NPT are treated with 1 μg/ml LPS and/or sample for 16 hours. As the reporter, SEAP activity in the cell-free culture media is measured as followed. Single cell-derived stable transfectants are plated in 5 ml of T-25 flask, and the media is decanted 24 h later. At this time, cells are washed twice with phosphate-buffered saline, and incubations are initiated by addition of new media. Chemicals are added to the culture medium after 24 h of incubations. Aliquots (25 ml) of medium from a control or chemical-treated cultures are taken at 0, 3, 20, 24, 48, and 72 h, heated at 65°C for 5 min to eliminate the alkaline phosphatase activity, and used immediately or stored at -20°C. Mixtures consisting of dilution buffer (25 ml), assay buffer (97 ml), culture media (25 ml), and 4-methylumbelliferyl phosphate (MUP, 1 mM, 3 ml) in each well of the 96-well plates are incubated for 60 min in the dark at room temperature. Fluorescence emits the product of the SEAP/MUP is measured at 449 nm using a 96-well plate fluorometer after excitation at 360 nm. |
细胞实验 | Macrophages RAW 264.7 are incubated with various concentrations of JSH-23 compound for 24 h. The cells are treated with WST-1 solution and absorbance is measured at 450 nm.(Only for Reference) |
分子量 | 240.34 |
分子式 | C16H20N2 |
CAS No. | 749886-87-1 |
store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 45 mg/mL (187.2 mM)
H2O: < 1 mg/mL (insoluble or slightly soluble)
Ethanol: 16 mg/mL (66.6 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO / Ethanol | 1 mM | 4.1608 mL | 20.8039 mL | 41.6077 mL | 104.0193 mL |
5 mM | 0.8322 mL | 4.1608 mL | 8.3215 mL | 20.8039 mL | |
10 mM | 0.4161 mL | 2.0804 mL | 4.1608 mL | 10.4019 mL | |
20 mM | 0.208 mL | 1.0402 mL | 2.0804 mL | 5.201 mL | |
50 mM | 0.0832 mL | 0.4161 mL | 0.8322 mL | 2.0804 mL | |
DMSO | 100 mM | 0.0416 mL | 0.208 mL | 0.4161 mL | 1.0402 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
JSH-23 749886-87-1 NF-Κb NF-κB Nuclear factor-κB Nuclear factor-kappaB Inhibitor JSH 23 JSH23 inhibit inhibitor