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JNK-IN-7

JNK-IN-7

产品编号 T3598   CAS 1408064-71-0
别名: JNK inhibitor

JNK-IN-7 (JNK inhibitor) 是 JNK 抑制剂,抑制 JNK1、JNK2和 JNK3,IC50分别为 1.5、2 和 0.7 nM。

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JNK-IN-7 Chemical Structure
JNK-IN-7, CAS 1408064-71-0
规格 价格/CNY 货期 数量
1 mg ¥ 865 现货
2 mg ¥ 1,470 现货
5 mg ¥ 2,190 现货
10 mg ¥ 2,850 现货
25 mg ¥ 4,780 现货
50 mg ¥ 6,830 现货
100 mg ¥ 9,260 现货
1 mL * 10 mM (in DMSO) ¥ 2,380 现货
产品目录号及名称: JNK-IN-7 (T3598)
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纯度: 99.58%
纯度: 99.53%
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生物活性
化学信息
存储 & 溶解度
参考文献
产品描述 JNK-IN-7 (JNK inhibitor) is a selective JNK1/2/3 inhibitor (IC50: 1.54/1.99/0.75 nM). It can also inhibit phosphorylation of c-Jun, which is a substrate of JNK kinase.
靶点活性 JNK3:0.75 nM, JNK1:1.54 nM, JNK2:1.99nM
体外活性 JNK-IN-7 is a relatively selective JNK inhibitor in cells. In addition to JNK 1, 2, 3, JNK-IN-7 also binds to IRAK1(IC50=14.1 nM), YSK4 (IC50=4.8 nM), ERK3 (IC50=22 nM), PIK3C3, PIP5K3 and PIP4K2C[1]. Expression of divalent metal-ion transporter 1 (DMT1) in HCT116 is demonstrated to be markedly decreased under stimulation with TNF for 24 and 48?h, while JNK-IN-7 can significantly reverse the decrease. TNF can down-regulate DMT1 expression, while JNK-IN-7 can markedly suppress this function[2].
激酶实验 A375 cells are pre-treated with 1 μM JNK-IN-7 for the indicated amounts of time. Remove the medium and wash 3 times with PBS. Resuspend the cell pellet with 1 mL Lysis Buffer (1% NP-40, 1% CHAPS, 25 mM Tris, 150 mM NaCl, Phosphatase Inhibitor Cocktail). Rotate end-to-end for 30 min at 4°C. Lysates are cleared by centrifugation at 14000 rpm for 15 min in the Eppendorf. The cleared lysates gel filtered into Kinase Buffer (0.1% NP-40, 20 mM HEPES, 150 mM NaCl, Phosphatase Inhibitor Cocktail, Protease Inhibitor Cocktail) using Bio-Rad 10DG colums. The total protein concentration of the gel-filtered lysate should be around 5-15 mg/mL. Cell lysate is labeled with the probe at 5 μM for 1 hour. Samples are reduced with DTT, and cysteines are blocked with iodoacetamide and gel filtered to remove excess reagents and exchange the buffer. Add 1 volume of 2X Binding Buffer (2% Triton-100, 1% NP-40, 2 mM EDTA, 2X PBS) and 50 μL streptavidin bead slurry and rotate end-to-end for 2 hours, centrifuge at 7000 rpm for 2 min. Wash 3 times with 1X Binding Buffer and 3 times with PBS. Add 30 μL 1X sample buffer to beads, heat samples at 95°C for 10 min. Run samples on an SDS-PAGE gel at 110V. After transferred, the membrane is immunoblotted with JNK antibody[1].
细胞实验 JNK-IN-7 is prepared in DMSO and stored, and then diluted with appropriate medium before use[2]. Intestinal epithelial cell line (HCT116) is cultured in DMEM medium, supplemented with 10% heat-inactivated fetal bovine serum (FBS), penicillin (100?U/mL) and streptomycin (100?g/mL), 2?mM L-gentamycin, and 50?μM 2-ME. These cells are stimulated with TNF (20?ng/mL), LPS (100?ng/mL), and IFN-γ (20?ng/mL), respectively. After 24 or 48?h of culture, cells are harvested followed by extraction of total RNA, and the levels of DMT1 mRNA are analyzed by qRT-PCR. To determine the mechanisms of TNF involved in regulating DMT1 expression, JNK-IN-7 (1?μM), NF-κB inhibitor (BAY 11-7082, 1?μM), and caspase-3/8 inhibitor (Z-DEVD-FMK, 50?μM) are also added into the culture medium. After 48?h of culture, cells are then collected to detect the expression of DMT1 by qRT-PCR[2].
别名 JNK inhibitor
分子量 493.56
分子式 C28H27N7O2
CAS No. 1408064-71-0

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

DMSO: 55 mg/mL (111.44 mM)

溶液配制表

可选溶剂 浓度 体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.0261 mL 10.1305 mL 20.261 mL 50.6524 mL
5 mM 0.4052 mL 2.0261 mL 4.0522 mL 10.1305 mL
10 mM 0.2026 mL 1.013 mL 2.0261 mL 5.0652 mL
20 mM 0.1013 mL 0.5065 mL 1.013 mL 2.5326 mL
50 mM 0.0405 mL 0.2026 mL 0.4052 mL 1.013 mL
100 mM 0.0203 mL 0.1013 mL 0.2026 mL 0.5065 mL

计算器

摩尔浓度计算器
稀释计算器
配液计算器
分子量计算器
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输入分子式,点击计算,可计算出产品的分子量。

参考文献

1. Zhang T, et al. Discovery of Potent and Selective Covalent Inhibitors of JNK. Chem Biol. 2012 Jan 27;19(1):140-54. 2. Wu W, et al. Divalent metal-ion transporter 1 is decreased in intestinal epithelial cells and contributes to the anemia in inflammatory bowel disease. Sci Rep. 2015 Nov 17;5:16344. 3. Xu P, Zeng S, Xia X, et al. FAM172A promotes follicular thyroid carcinogenesis and may be a marker of FTC[J]. Endocrine-Related Cancer. 2020, 1(aop).

文献引用

1. Xu P, Zeng S, Xia X, et al. FAM172A promotes follicular thyroid carcinogenesis and may be a marker of FTC. Endocrine-Related Cancer. 2020, 1(aop)
Pinusolide JNK-IN-8 Shizukaol B Ergosterol peroxide D-JNKI-1 acetate BI-78D3 Medicarpin Lappaol A

相关化合物库

该产品包含在如下化合物库中:
抗癌药物库 抗癌临床化合物库 神经退行性疾病化合物库 抗糖尿病库 MAPK 抑制剂库 抗胰腺癌化合物库 抑制剂库 抗卵巢癌化合物库 已知活性化合物库 HIF-1化合物库

剂量换算

对于不同动物的给药剂量换算,您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。

体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。

第一步:请输入动物实验的基本信息
剂量
mg/kg
每只动物体重
g
给药体积
μL
动物数量
第二步:请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
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计算 重置

技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

JNK-IN-7 1408064-71-0 MAPK JNK Inhibitor JNK inhibitor inhibit JNK IN 7 JNKIN7 inhibitor

 

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