store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Isosilybin A 是从水飞蓟中分离出来的一种黄酮木脂素,可抑制癌细胞增殖并诱导 G1 期停滞和凋亡,通过靶向 Akt-NF-κB-雄激素受体轴激活前列腺癌细胞的凋亡机制,具有抗前列腺癌活性。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 1,660 | 现货 | ||
5 mg | ¥ 2,890 | 现货 | ||
10 mg | ¥ 4,220 | 现货 | ||
25 mg | ¥ 6,590 | 现货 | ||
50 mg | ¥ 8,890 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 2,960 | 现货 |
产品描述 | Isosilybin A is a partial PPARγ agonist, it significantly induced ABCA1 protein expression, it inhibited both monophenolase (IC50=1.7-7.6μM) and diphenolase (IC50=12.1-44.9μM) of tyrosinase. Isosilybin A shows anti-angiogenic efficacy, it has anti-prostate cancer (PCA) activity that is mediated via cell cycle arrest and apoptosis induction. |
体外活性 | To investigate the effects of milk thistle extract and its main flavonolignans (silybin A, silybin B, Isosilybin A and isosilybin B) on CYP2C8 activity at relevant concentrations, the effect of milk thistle extract and the flavonolignans on CYP2C8 enzyme activity was studied in vitro using human liver microsomes (HLM) incorporating an enzyme-selective substrate for CYP2C8, amodiaquine. Metabolite formation was analyzed using liquid chromatography-tandem mass spectrometry (LC/MS-MS). The concentration causing 50% inhibition of enzyme activity (IC50) was used to express the degree of inhibition. Isosilibinin, a mixture of the diastereoisomers Isosilybin A and isosilybin B, was found to be the most potent inhibitor, followed by isosilybin B with IC50 values (mean ± SE) of 1.64 ± 0.66 μg/mL and 2.67 ± 1.18 μg/mL, respectively. The rank order of observed inhibitory potency after isosilibinin was silibinin > Isosilybin A > silybin A > milk thistle extract > and silybin B[1] |
别名 | 异水飞蓟素 A, 异水飞蓟宾A, 水飞蓟素 B2 |
分子量 | 482.44 |
分子式 | C25H22O10 |
CAS No. | 142796-21-2 |
store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 90 mg/mL(186.55 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.0728 mL | 10.364 mL | 20.728 mL | 51.8199 mL |
5 mM | 0.4146 mL | 2.0728 mL | 4.1456 mL | 10.364 mL | |
10 mM | 0.2073 mL | 1.0364 mL | 2.0728 mL | 5.182 mL | |
20 mM | 0.1036 mL | 0.5182 mL | 1.0364 mL | 2.591 mL | |
50 mM | 0.0415 mL | 0.2073 mL | 0.4146 mL | 1.0364 mL | |
100 mM | 0.0207 mL | 0.1036 mL | 0.2073 mL | 0.5182 mL |
中药材名称 | 中药材拉丁名 | 性 | 味 | 归经 |
水飞蓟 | Silybum marianum (L.)Gaertn.[Carduus marianus L.] | 凉 | 苦 | 肝, 胆 |
中成药名称 | 处方组成 | 主治疾病 | 中成药类型 |
当飞利肝宁胶囊 | 水飞蓟,当药 | 口服,一次4粒,一日3次或遵医嘱。 | 化湿药 |
参芪肝康片 | 茵陈,党参,水飞蓟,五味子,当归,党参,刺五加浸膏 | 肌内注射,一次2-4ml,一日4-6ml。静脉滴注,一次20-100ml,用5-10%葡萄糖注射液稀释后应用,或遵医嘱。 | 扶正药 |
当飞利肝宁片 | 水飞蓟,当药 | 口服。一次2片,一日3次或遵医嘱,小儿酌减。 | 清热药 |
五子降脂胶囊 | 蛇床子,五灵脂,丹参,赤芍,蒲黄(生),淫羊藿,菟丝子,浮萍,石菖蒲,泽泻,金樱子,枸杞子,五味子,何首乌,水飞蓟 | 口服,一次5粒,一日3次。8周为一疗程。 | 扶正药 |
参芪肝康胶囊 | 当归,党参,水飞蓟,五味子,茵陈,黄芪,刺五加浸膏 | 口服,一次5粒,一日3次。 | 扶正药 |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Isosilybin A 142796-21-2 Apoptosis DNA Damage/DNA Repair Membrane transporter/Ion channel Metabolism Proteases/Proteasome Tyrosinase ABC PPAR 异水飞蓟素 A inhibit Inhibitor 异水飞蓟宾A 水飞蓟素 B2 inhibitor