Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Isosilybin (Isosilibinin) 是一种类黄酮,来源于乳蓟;能够抑制CYP3A4诱导(IC50:74 μM)。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 247 | 现货 | ||
5 mg | ¥ 690 | 现货 | ||
10 mg | ¥ 995 | 现货 | ||
25 mg | ¥ 2,320 | 现货 | ||
50 mg | ¥ 3,530 | 现货 | ||
100 mg | ¥ 5,120 | 待询 | ||
1 mL * 10 mM (in DMSO) | ¥ 748 | 现货 |
产品描述 | Isosilybin (Isosilibinin) and Silybin might be suitable candidates to design potent PXR antagonists to prevent drug-drug interactions via CYP3A4 in cancer patients. |
靶点活性 | CYP3A4:74 μM. |
体外活性 | The reporter gene assay shows that milk thistle’s components silybin and isosilybin are responsible for the inhibition of PXR-mediated CYP3A4 induction by milk thistle. The isomer isosilybin is a stronger inhibitor of PXR-mediated CYP3A4 induction compared with silybin. A solution of 89, 133, and 200 μM isosilybin significantly inhibits CYP3A4 induction by 64, 82, and 88%, respectively. Isosilybin inhibits CYP3A4 induction with an IC50 of 74 μM[1]. Isosilybin B and isosilybin A, two diastereoisomers isolated from silymarin, have anti-prostate cancer (PCA) activity that is mediated via cell cycle arrest and apoptosis induction. Isosilybin B and isosilybin A treatment results in growth inhibition and cell death together with a strong G(1) arrest and apoptosis in human prostate cancer LNCaP and 22Rv1 cells[2]. Isosilybin B causes increased phosphorylation of Akt (Ser-473 and Thr-308) and Mdm2 (Ser-166), which is linked with androgen receptor degradation as pretreatment with PI3K inhibitor (LY294002)-restored androgen receptor level. Isosilybin B treatment enhances the formation of complex between Akt, Mdm2 and AR, which promotes phosphorylation-dependent AR ubiquitination and its degradation by proteasome[3]. Isosilybin A is able to significantly activate PPARγ at a concentration of 30 μM (2.08±0.48 fold, p<0.01). Isosilybin A causes transactivation of a PPARγ-dependent luciferase reporter in a concentration-dependent manner. In silico docking studies suggests a binding mode for 3 distinct from that of the inactive silymarin constituents, with one additional hydrogen bond to Ser342 in the entrance region of the ligand-binding domain of the receptor[4]. |
细胞实验 | LNCaP cells and 22Rv1 cells are plated and treated at 40–50% confluency with different doses of isosilybin B and isosilybin A (10–90 μM in medium) dissolved originally in Dimethyl sulfoxide (DMSO) for the desired time periods (24–48 h) in serum condition. An equal amount of DMSO (vehicle) is present in each treatment, including control; DMSO concentration did not exceed 0.1% (v/v) in any treatment. At the end of desired treatments, total cell number is determined by counting each sample in duplicate using a hemocytometer under an inverted microscope. Cell viability is determined using trypan blue exclusion method[2]. |
别名 | Isosilybinin, Isosilibinin, 异水飞蓟宾, Silymarin, Silybin B, Q-100795 |
分子量 | 482.44 |
分子式 | C25H22O10 |
CAS No. | 72581-71-6 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 88 mg/mL(182.4 mM)
Chloroform, Dichloromethane, Ethyl Acetate: Soluble
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.0728 mL | 10.364 mL | 20.728 mL | 51.8199 mL |
5 mM | 0.4146 mL | 2.0728 mL | 4.1456 mL | 10.364 mL | |
10 mM | 0.2073 mL | 1.0364 mL | 2.0728 mL | 5.182 mL | |
20 mM | 0.1036 mL | 0.5182 mL | 1.0364 mL | 2.591 mL | |
50 mM | 0.0415 mL | 0.2073 mL | 0.4146 mL | 1.0364 mL | |
100 mM | 0.0207 mL | 0.1036 mL | 0.2073 mL | 0.5182 mL |
中药材名称 | 中药材拉丁名 | 性 | 味 | 归经 |
水飞蓟 | Silybum marianum (L.)Gaertn.[Carduus marianus L.] | 凉 | 苦 | 肝, 胆 |
中成药名称 | 处方组成 | 主治疾病 | 中成药类型 |
当飞利肝宁片 | 水飞蓟,当药 | 口服。一次2片,一日3次或遵医嘱,小儿酌减。 | 清热药 |
参芪肝康片 | 茵陈,党参,水飞蓟,五味子,当归,党参,刺五加浸膏 | 肌内注射,一次2-4ml,一日4-6ml。静脉滴注,一次20-100ml,用5-10%葡萄糖注射液稀释后应用,或遵医嘱。 | 扶正药 |
五子降脂胶囊 | 蛇床子,五灵脂,丹参,赤芍,蒲黄(生),淫羊藿,菟丝子,浮萍,石菖蒲,泽泻,金樱子,枸杞子,五味子,何首乌,水飞蓟 | 口服,一次5粒,一日3次。8周为一疗程。 | 扶正药 |
参芪肝康胶囊 | 当归,党参,水飞蓟,五味子,茵陈,黄芪,刺五加浸膏 | 口服,一次5粒,一日3次。 | 扶正药 |
当飞利肝宁胶囊 | 水飞蓟,当药 | 口服,一次4粒,一日3次或遵医嘱。 | 化湿药 |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Isosilybin 72581-71-6 Metabolism P450 Isosilybinin Cytochrome P450 Isosilibinin Inhibitor Q 100795 inhibit CYPs 异水飞蓟宾 Silymarin Silybin B Q100795 Q-100795 inhibitor