Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Isatin (2,3-Indolinedione) 是一种单胺氧化酶抑制剂,IC50为3 μM。它也可作为心钠肽刺激和一氧化氮刺激的鸟苷酸环化酶活性的拮抗剂,显示对血清素系统有影响。
产品描述 | Isatin (2,3-Indolinedione) is an endogenous MAO inhibitor. |
靶点活性 | MAO-A:58 μM, MAO-B:14 μM, MAO:15 μM |
体外活性 | Isatin induces apoptosis of MCF-7 cells. Bcl-2 expression is decreased and the ratio of Bcl-2 to Bax is significantly decreased by isatin. The mitochondrial transmembrane potential is markedly decreased and the release of cytochrome c into the cytosol is elevated following treatment with isatin. At the same time, caspase-9 and -3 are stimulated, followed by the degradation of ICAD, a caspase-3 substrate. Isatin and its analogs inhibits the proliferation of some cancer cells, including colon HT29, breast MCF-7, lung A549 and melanoma UACC903 cells and is a dual inhibitor of tubulin polymerization and the Akt pathway[4]. |
体内活性 | Isatin is an endogenous indole that is increased in stress, inhibits monoamine oxidase (MAO) B and improves bradykinesia and striatal dopamine levels in rat models of Parkinson's disease. Isatin has a distinct and discontinuous distribution in rat brain and other tissues; the highest concentrations in the brain are found in the hippocampus and cerebellum. In rodent models isatin has been shown to cause a widespectrum of dose-dependent physiological and biological actions, such as anxiogenic and sedative effects, memory dysfunction and inhibition of food and water intake. Significantly, isatin readily crosses the blood-brain barrier so that a peritoneal dose of 100 mg/kg would result in a concentration of about 120 μM in the rat brain. This concentration would increase further with repeated injections[2]. |
细胞实验 | Cell viability was estimated by a colorimetric method, which is based on the ability of cellular dehydrogenases of viable cells to reduce MTT from a yellow watersoluble dye to a dark blue insoluble formazan product. SHSY5Y cells were seeded in 96-well plates at 4×104 cells/well/(100 ml) and allowed to attach. The cells were then treated with isatin and returned to the incubator for 24 or 48 h. MTT 25 μl (5 mg/ml) was added to all wells and allowed to incubate in the dark at 37℃ for 2 h followed by cell lysis. The plates were read with an OPTImax microplate reader at wavelength of 562 nm. Controls included untreated cells and medium alone, with all MTT assays performed in triplicate.(Only for Reference) |
别名 | Pseudoisatin, 板蓝根, Indoline-2,3-dione, 2,3-Indolinedione |
分子量 | 147.13 |
分子式 | C8H5NO2 |
CAS No. | 91-56-5 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: 10 mg/mL (67.96 mM)
H2O: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 28 mg/mL (190.3 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
Ethanol / DMSO | 1 mM | 6.7967 mL | 33.9836 mL | 67.9671 mL | 169.9178 mL |
5 mM | 1.3593 mL | 6.7967 mL | 13.5934 mL | 33.9836 mL | |
10 mM | 0.6797 mL | 3.3984 mL | 6.7967 mL | 16.9918 mL | |
20 mM | 0.3398 mL | 1.6992 mL | 3.3984 mL | 8.4959 mL | |
50 mM | 0.1359 mL | 0.6797 mL | 1.3593 mL | 3.3984 mL | |
DMSO | 100 mM | 0.068 mL | 0.3398 mL | 0.6797 mL | 1.6992 mL |
中药材名称 | 中药材拉丁名 | 性 | 味 | 归经 |
板蓝根 | Isatis Indigotica Fort. | 寒 | 苦 | 心, 胃 |
中成药名称 | 处方组成 | 主治疾病 | 中成药类型 |
板蓝根颗粒 | 板蓝根 | 一次半袋-1袋,一日3-4次。 | 清热药 |
柴银感冒颗粒 | 柴胡,金银花,拳参,射干,僵蚕,大青叶,板蓝根,陈皮,甘草 | 开水冲服,一次1~2袋,一日3次。 | 清热药 |
儿感退热宁颗粒 | 青蒿,板蓝根,连翘,菊花,苦杏仁,桔梗,薄荷,甘草 | 口服,十岁以上儿童一次5~7.5g,五岁至十岁儿童一次3~5g,三岁至五岁儿童一次2~3g,一日3次。 | 解表药 |
板蓝根口服液 | 板蓝根 | 口服,一次1支,一日4次。 | 清热药 |
板蓝根含片 | 板蓝根 | 含服,一次4~5片,一日4次。 | 清热药 |
柴黄清热颗粒 | 石膏,柴胡,知母,黄芩,大青叶,薄荷,连翘,牛黄,板蓝根,水牛角浓缩粉,芦根 | 开水冲服,一次20g,一日2~3次。 | 解表药 |
碧云砂乙肝颗粒 | 白花蛇舌草,茜草,板蓝根,虎杖,青黛,绿茶,紫草,土茯苓,佛手,山楂,鸡内金,蚕砂,丹参,重楼,灵芝,麦冬,五味子,白芍,白矾 | 开水冲服。一次10g,一日3次。 | 清热药 |
板蓝根滴眼液 | 板蓝根 | 滴入眼睑内。一次1~2滴,一日6次。疗程7天。 | 清热药 |
二丁胶囊 | 紫花地丁,蒲公英,板蓝根,半边莲 | 口服。一次5粒,一日3次。 | 清热药 |
柴石退热颗粒 | 柴胡,黄芩,石膏,青蒿,板蓝根,金银花,大黄,蒲公英,知母,连翘 | 开水冲服,每次1袋,一日4次。 | 清热药 |
板蓝根胶囊 | 板蓝根 | 口服,一次4粒,一日4次。 | 清热药 |
板蓝根滴丸 | 板蓝根 | 口服。一次20~40丸,一日3次,或遵医嘱。 | 清热药 |
板蓝根软胶囊 | 板蓝根 | 口服,一次4粒,一日4次。 | 清热药 |
板蓝根咀嚼片 | 板蓝根 | 口服,一次4片,一日4次。 | 清热药 |
板蓝根分散片 | 板蓝根 | 口服、一次2~4片,一日3次。 | 清热药 |
儿童清肺口服液 | 麻黄,苦杏仁(去皮炒),石膏,甘草,桑白皮(蜜炙),瓜蒌皮,黄芩,板蓝根,法半夏,浙贝母,橘红,紫苏子(炒),葶苈子,紫苏叶,细辛,薄荷,枇杷叶(蜜炙),白前,前胡,石菖蒲,天花粉,青礞石(煅) | 口服,一次2支,六岁以下一次1支,一日3次。 | 清热药 |
板蓝根茶 | 板蓝根 | 开水冲服,一次1块,一日3次。 | 清热药 |
板蓝根颗粒(无糖型) | 板蓝根 | 口服,一次1-2袋,一日3-4次。 | 清热药 |
板蓝根泡腾片 | 板蓝根 | 加热水适量,泡腾溶解后口服。一次0.5-1片,一日3-4次。 | 清热药 |
板蓝大青片 | 板蓝根,大青叶 | 口服。每次4片,一日3次。预防流感、乙脑,一日4。 | 清热药 |
方剂名称 | 处方组成 | 剂型 | 处方来源 |
解毒丸6 | 板蓝根,贯众,青黛,甘草 | 丸剂 | 《三因》卷十。 |
解毒丸8 | 滑石,黄芩,贯众,茯苓,山栀子,干姜,龙胆,大豆,青黛,甘草,薄荷,寒水石,益智仁,缩砂仁,大黄,山豆根,生地黄,桔梗,百药煎,紫河车,粉花,马勃,板蓝根,黄药子 | 丸剂 | 《洁古家珍》。 |
解毒丸3 | 山豆根,板蓝根,甘草,山慈姑,马鬃,续随子,黄药子,大黄,紫河车,木通,芒硝,五味子,藿香,寒水石,雄黄,贯众,白僵蚕,葛根,茜草根,薄荷,绿豆,百药煎,朱砂,麝香 | 丸剂 | 《普济方》卷二五一。 |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Isatin 91-56-5 Apoptosis Metabolism Neuroscience MAO Monoamine Oxidase Inhibitor Pseudoisatin 板蓝根 Indoline-2,3-dione 2,3-Indolinedione inhibit inhibitor