Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Importazole 是一种核转运受体 importin-β 小分子抑制剂。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 196 | 现货 | ||
5 mg | ¥ 438 | 现货 | ||
10 mg | ¥ 611 | 现货 | ||
25 mg | ¥ 1,370 | 现货 | ||
50 mg | ¥ 2,480 | 现货 | ||
100 mg | ¥ 3,760 | 现货 | ||
200 mg | ¥ 5,380 | 现货 | ||
500 mg | ¥ 8,360 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 437 | 现货 |
产品描述 | Importazole is an inhibitor of the nuclear transport receptor importin-β. |
体外活性 | Importazole is an effective inhibitor of the Ran/importin-β interaction in vitro. Importazole specifically blocks importin-β-mediated nuclear import both in Xenopus egg extracts and cultured cells, without disrupting transportin-mediated nuclear import or CRM1-mediated nuclear export. When added during mitosis, importazole impairs the release of an importin-β cargo FRET probe and causes both predicted and novel defects in spindle assembly[1]. It also inhibits proliferation and induces apoptosis of multiple myeloma cells by blocking the NF-κB signal pathway in vitro[2]. |
细胞实验 | For all import and export experiments, HEK 293 cells stably expressing NFAT-GFP are grown on glass coverslips to approximately 50% confluency prior to drug treatment. In all cases, importazole is used at 40 μM and leptomycin B is used at 10 ng/ml. For controls, DMSO is used at a concentration of 0.4%. Ionomycin is added at 1.25 μM. Importazole and leptomycin B treatments are all for 1 hour. In all experiments cells are fixed with 4% formaldehyde prior to fluorescence microscopy. DNA is visualized with 1 μg/ml Hoechst dye. For quantification, 100 cells from each condition are analyzed and the percentage that shows nuclear accumulation of NFAT-GFP calculated.(Only for Reference) |
分子量 | 318.42 |
分子式 | C20H22N4 |
CAS No. | 662163-81-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: 28 mg/mL(87.9 mM)
DMSO: 12.5 mg/mL (39.26 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
Ethanol / DMSO | 1 mM | 3.1405 mL | 15.7025 mL | 31.4051 mL | 78.5127 mL |
5 mM | 0.6281 mL | 3.1405 mL | 6.281 mL | 15.7025 mL | |
10 mM | 0.3141 mL | 1.5703 mL | 3.1405 mL | 7.8513 mL | |
20 mM | 0.157 mL | 0.7851 mL | 1.5703 mL | 3.9256 mL | |
Ethanol | 50 mM | 0.0628 mL | 0.3141 mL | 0.6281 mL | 1.5703 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Importazole 662163-81-7 Apoptosis Others Inhibitor inhibit inhibitor