Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Idoxuridine (Dendrid) 是一种抑制病毒 DNA 合成的脱氧尿苷类似物,用作抗病毒剂。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
500 mg | ¥ 172 | 现货 | ||
1 g | ¥ 272 | 现货 | ||
5 g | ¥ 622 | 现货 | ||
10 g | ¥ 913 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 189 | 现货 |
产品描述 | Idoxuridine (Dendrid) is an analog of DEOXYURIDINE that inhibits viral DNA synthesis. The drug is used as an antiviral agent. |
靶点活性 | FHV-1:4.3 μM |
体外活性 | Idoxuridine is a nucleoside analogue, a modified form of deoxyuridine, similar enough to be incorporated into viral DNA replication, but the iodine atom added to the uracil component blocks base pairing.Idoxuridine is used for topical treatment of herpetic eye infections due to herpes simplex virus (HSV)[2]. |
细胞实验 | The CRFK cells are cultured in 25-cm2 flasks that contained DMEM with 10% FBS. The concentration of idoxuridine is 5, 10, and 50 μM. Cells from 1 control flask and 1 flask containing each drug concentration are examined by use of an inverted microscope to detect morphologic changes and evaluate confluence, and cells are then harvested at 24, 48, and 72 hours. Culture medium is decanted, and the monolayer is rinsed once with PBS solution. Cells are then enzymatically detached with trypsin-EDTA solution. Trypsinization is stopped by the addition of DMEM with 10% FBS, and cells are forcefully pipetted to ensure complete detachment from the flasks. Each flask is then rinsed once with DMEM with 10% FBS, and the eluent is combined with the first cell suspension to ensure collection of as many cells as possible from each flask. Cell-containing medium is centrifuged (300 X g for 6 minutes), and the supernatant is decanted. Cells are then resuspended in a known volume of DMEM and counted on a hemacytometer. Cells cultured in each drug concentration at each time point are counted twice, and the total number of cells in each flask is calculated. (Only for Reference) |
别名 | IDU, 碘苷, SKF 14287, 5-Iodo-2′-deoxyuridine, NSC 39661, 5-IUdR, Dendrid |
分子量 | 354.1 |
分子式 | C9H11IN2O5 |
CAS No. | 54-42-2 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 33 mg/mL (93.2 mM)
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.8241 mL | 14.1203 mL | 28.2406 mL | 70.6015 mL |
5 mM | 0.5648 mL | 2.8241 mL | 5.6481 mL | 14.1203 mL | |
10 mM | 0.2824 mL | 1.412 mL | 2.8241 mL | 7.0602 mL | |
20 mM | 0.1412 mL | 0.706 mL | 1.412 mL | 3.5301 mL | |
50 mM | 0.0565 mL | 0.2824 mL | 0.5648 mL | 1.412 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Idoxuridine 54-42-2 Microbiology/Virology Antifection HSV inhibit SKF-14287 Feline herpesvirus NSC-39661 Orthopoxvirus Iodinated thymidine analogue SKF14287 DNA polymerase IDU Inhibitor 碘苷 SKF 14287 5-Iodo-2′-deoxyuridine Phosphatase IdUrd Herpesviruses Phosphorylases inhibitor Antiviral agents NSC 39661 NSC39661 5-IUdR Dendrid inhibitor