Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ibuprofen (Advil) 是一种丙酸衍生物和非甾体抗炎药 (NSAID),具有抗炎、镇痛和解热作用。它是COX-1和COX-2的抑制剂,IC50值分别为 13 和 370 μM,导致前列腺素和血栓素前体的形成减少。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
500 mg | ¥ 333 | 现货 | ||
1 g | ¥ 418 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 418 | 现货 |
产品描述 | Ibuprofen (Advil) is a propionic acid derivate and nonsteroidal anti-inflammatory drug (NSAID) with anti-inflammatory, analgesic, and antipyretic effects. Ibuprofen inhibits the activity of cyclo-oxygenase I and II, resulting in a decreased formation of precursors of prostaglandins and thromboxanes. |
靶点活性 | COX-2:370 μM, COX-1:13 μM |
体外活性 | 在HRHF方式诱导的成年雌性Sprague-Dawley大鼠模型中,Ibuprofen通过阻断血清C1和2C以及胶原降解与合成,治疗关节炎症和早期关节软骨退变. |
体内活性 | 在细胞中,Ibuprofen能够抑制环氧合酶COX-1和COX-2,及花生四烯酸转换为前列腺素H2 (PGH2)。在前列腺肿瘤细胞,Ibuprofen能够抑制NF-κB和IKKα的激活。 |
激酶实验 | Radiochemical enzyme assays for COX-1 and COX-2: 10 μL of purified COX-1 (0.7-0.8 μg) or COX-2 (3.0 units, 0.3μg) is activated with 50 μL of cofactor solution [l-epinephrine (1.3 mg/mL), reduced glutathione (0.3 mg/mL), and hematin (1.3 mg/mL) in oxygen-free Tris-HCl buffer (pH 8.0)]. The enzyme solution (60 μL) is added to Ibuprofen solutions or DMSO (20 μL) after [14C]arachidonic acid is added in 0.2 mL eight-strip test tubes and preincubated 10 minutes on ice. Samples are incubated for 15 minutes at 37 °C, after which the reaction is terminated by addition of 10 μL of 2 M HCl and 5 μL of carrier solution (PGE2 and PGF2α, 0.2 μg/mL of each in EtOH). The unmetabolized arachidonic acid is separated from the prostaglandin products by column chromatography and eluted with n-hexane-dioxane-glacial acetic acid (70:30:1). The prostaglandin products are then eluted with EtOAc-MeOH (85:15), and the samples are counted in a Packard scintillation spectrometer. IC50 values are obtained by linear regression analysis. |
细胞实验 | Each cell line is incubated with Ibuprofen of various concentrations for 48 hours. Cell survival is estimated by the MTT assay, and cell death is determined by Hoechst staining used to distinguish between intact cell nuclei and fragmented nuclei undergoing cell death. Cells are lysed and analyzed by western blotting for detection of the p75NTR protein.(Only for Reference) |
别名 | Brufen, (±)-Ibuprofe, 布洛芬, Motrin, Advil |
分子量 | 206.28 |
分子式 | C13H18O2 |
CAS No. | 15687-27-1 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 39 mg/mL (189.1 mM)
Ethanol: 39 mg/mL (189.1 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO / Ethanol | 1 mM | 4.8478 mL | 24.2389 mL | 48.4778 mL | 121.1945 mL |
5 mM | 0.9696 mL | 4.8478 mL | 9.6956 mL | 24.2389 mL | |
10 mM | 0.4848 mL | 2.4239 mL | 4.8478 mL | 12.1194 mL | |
20 mM | 0.2424 mL | 1.2119 mL | 2.4239 mL | 6.0597 mL | |
50 mM | 0.097 mL | 0.4848 mL | 0.9696 mL | 2.4239 mL | |
100 mM | 0.0485 mL | 0.2424 mL | 0.4848 mL | 1.2119 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Ibuprofen 15687-27-1 Immunology/Inflammation Neuroscience COX AGS gastric cancer Brufen inhibit photosensitivity Cyclooxygenase angiogenesis microtubule breast cancer Apoptosis (±)-Ibuprofe (±)-Ibuprofen T cells Neuroprotection 布洛芬 anti-tumor Motrin inflammation anti-cancer cystic fibrosis tumor microenvironment Ibuprofe Advil Inhibitor inhibitor