Powder: -20°C for 3 years | In solvent: -80°C for 1 year
ITE 是一种有效的芳烃受体 (AhR) 内源性激动剂,Ki 值为 3 nM。它具有免疫抑制作用。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 219 | 现货 | ||
2 mg | ¥ 313 | 现货 | ||
5 mg | ¥ 538 | 现货 | ||
10 mg | ¥ 863 | 现货 | ||
25 mg | ¥ 1,550 | 现货 | ||
50 mg | ¥ 2,590 | 现货 | ||
100 mg | ¥ 3,920 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 593 | 现货 |
产品描述 | ITE is a potent endogenous agonist of aryl hydrocarbon receptor (AhR) (Ki : 3 nM), has immunosuppressive activity. |
靶点活性 | AhR:3 nM (Ki) |
体外活性 | ITE dose- and time-dependently inhibited proliferation of HPAECs with a maximum inhibition of 83% at 20 μM after 6 days of treatment. ITE rapidly decreased AhR protein levels, while it increased mRNA levels of cytochrome P450 (CYP), family 1, member A1 (CYP1A1) and B1 (CYP1B1), indicating activation of the AhR/CYP1A1 and AhR/CYP1B1 pathways in HPAECs. The AhR siRNA significantly suppressed AhR protein expression, whereas it did not significantly alter ITE-inhibited growth of HPAECs[1]. |
体内活性 | ITE diminishes colitis pathology through induction of Tregs; reduces inflammatory cytokines, inflammation score, and macrophage frequency; and induces DCs resulting in amelioration of colitis. Therefore, nontoxic endogenous ITE promotes the induction of Tregs and may be useful for the treatment of IBD[2]. |
细胞实验 | Immunohistochemistry was performed to localize AhR expression in human lung tissues. The crystal violet method and MTT assay were used to determine ITE's effects on growth of HPAECs. The AhR activation in HPAECs was confirmed using Western blotting and RT-qPCR. The role of AhR in ITE-affected proliferation of HPAECs was assessed using siRNA knockdown method followed by the crystal violet method[1] |
动物实验 | At the start of DSS induction, mice received 100 μl by intraperitoneal injection of vehicle and ITE (10 mg/kg body wt) twice a week on each Monday and Thursday until week 6 at the end point of the experiment. During a pilot study, we used several (5, 10, 20, 40, and 80 mg/kg body wt) doses of ITE and noticed that the 10-mg/kg dose was the lowest dose giving maximum protection. Therefore, Used this dose in entire study. At the experimental end point blood was collected by tail-vein bleedings and serum was obtained following centrifugation. For comparison, a similar treatment was also given to normal BL/6 mice to see the effect of ITE alone[2]. |
分子量 | 286.31 |
分子式 | C14H10N2O3S |
CAS No. | 448906-42-1 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 41 mg/mL (143.20 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.4927 mL | 17.4636 mL | 34.9272 mL | 87.3179 mL |
5 mM | 0.6985 mL | 3.4927 mL | 6.9854 mL | 17.4636 mL | |
10 mM | 0.3493 mL | 1.7464 mL | 3.4927 mL | 8.7318 mL | |
20 mM | 0.1746 mL | 0.8732 mL | 1.7464 mL | 4.3659 mL | |
50 mM | 0.0699 mL | 0.3493 mL | 0.6985 mL | 1.7464 mL | |
100 mM | 0.0349 mL | 0.1746 mL | 0.3493 mL | 0.8732 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
ITE 448906-42-1 Immunology/Inflammation Metabolism AhR Aryl Hydrocarbon Receptor Inhibitor inhibit inhibitor