首页 工具
登录
购物车
ISCK03

ISCK03

产品编号 T3452   CAS 945526-43-2

ISCK03 是一种特异性的 SCF/c-Kit 抑制剂。

TargetMol的所有产品和服务仅用于科学研究,不能被用于人体,我们也不向个人提供产品和服务。
ISCK03 Chemical Structure
ISCK03, CAS 945526-43-2
规格 价格/CNY 货期 数量
2 mg ¥ 297 现货
5 mg ¥ 497 现货
10 mg ¥ 828 现货
25 mg ¥ 1,780 现货
50 mg ¥ 2,680 现货
100 mg ¥ 3,930 现货
1 mL * 10 mM (in DMSO) ¥ 547 现货
产品目录号及名称: ISCK03 (T3452)
点击图片重新获取验证码
选择批次  
更多批次查询请联系客服
生物活性
化学信息
存储 & 溶解度
参考文献
产品描述 ISCK03 is a selective SCF/c-Kit inhibitor.
体外活性 Pretreatment of 501mel cells with ISCK03 inhibits SCF-induced c-kit phosphorylation dose dependently. ISCK03 also inhibits p44/42 ERK mitogen-activated protein kinase (MAPK) phosphorylation, which is known to be involved in SCF/c-kit downstream signaling. However ISCK03 does not inhibit hepatocyte growth factor (HGF)-induced phosphorylation of p44/42 ERK proteins[1]. ISCK03, a tyrosine kinase inhibitor specific to KIT, prevents survival of CCDC26-KD cells under low-serum conditions. All treated cells exhibits sensitivity to ISCK03 in a dose-dependent manner. The survival of KD cells is suppressed to the same level as that of non-KD cells after ISCK03 treatment. On the contrary, ISCK03 treatment has limited effects on the growth of control K562 and KD clone 3–4 cells under high-serum concentration conditions[2].
体内活性 Oral administration of ISCK03 induces the dose-dependent depigmentation of newly regrown hair which is reversed with cessation of ISCK03 treatment. The topical application of ISCK03 promotes the depigmentation of UV-induced hyperpigmented spots. Fontana-Masson staining analysis shows epidermal melanin is diminished in spots treated with ISCK03[1].
激酶实验 ATP is dispensed into 384-well plates, chemical compounds (ISCK03: 2.5, 5, 10, 100 μM) are added by replicative plate, and recombinant human c-kit protein is added for the kinase reaction. Following a 45-min incubation at 37°C, the development reaction is carried out for 40 min at room temperature. After the reaction is stopped, the coumrain and fluorescein fluorescence-emission signals are detected[1].
细胞实验 To determine any cytotoxic effects of ISCK03 on 501mel cells, MTT assays are performed with various doses of ISCK03 (1, 5, 10 μM). 501mel cells are cultured with SCF alone (50 ng/mL) or SCF with ISCK03 for 48 h[1].
分子量 355.45
分子式 C19H21N3O2S
CAS No. 945526-43-2

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

DMSO: 50 mg/mL (140.66 mM)

溶液配制表

可选溶剂 浓度 体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.8133 mL 14.0667 mL 28.1334 mL 70.3334 mL
5 mM 0.5627 mL 2.8133 mL 5.6267 mL 14.0667 mL
10 mM 0.2813 mL 1.4067 mL 2.8133 mL 7.0333 mL
20 mM 0.1407 mL 0.7033 mL 1.4067 mL 3.5167 mL
50 mM 0.0563 mL 0.2813 mL 0.5627 mL 1.4067 mL
100 mM 0.0281 mL 0.1407 mL 0.2813 mL 0.7033 mL

计算器

摩尔浓度计算器
稀释计算器
配液计算器
分子量计算器
=
X
X
X
=
X
=
/
g/mol

输入分子式,点击计算,可计算出产品的分子量。

参考文献

1. Na YJ, et al. [4-t-butylphenyl]-N-(4-imidazol-1-yl phenyl)sulfonamide (ISCK03) inhibits SCF/c-kit signaling in 501mel human melanoma cells and abolishes melanin production in mice and brownish guinea pigs. Biochem Pharmacol. 2007 Sep 1;74(5):780-6. 2. Hirano T, et al. Long noncoding RNA, CCDC26, controls myeloid leukemia cell growth through regulation of KIT expression. Mol Cancer. 2015 Apr 19;14:90. doi: 10.1186/s12943-015-0364-7.
Dasatinib Regorafenib Cabozantinib hydrochloride Imatinib JNK-IN-8 Tandutinib Labuxtinib CHMFL-KIT-033

相关化合物库

该产品包含在如下化合物库中:
酪氨酸激酶分子库 免疫/炎症分子化合物库 已知活性化合物库 经典已知活性库 抑制剂库 NO PAINS 化合物库 干细胞分化化合物库 表型筛选靶点鉴定库 激酶抑制剂库

剂量换算

对于不同动物的给药剂量换算,您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。

体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。

第一步:请输入动物实验的基本信息
剂量
mg/kg
每只动物体重
g
给药体积
μL
动物数量
第二步:请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
% DMSO
%
% Tween 80
% ddH2O
计算 重置

技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

ISCK03 945526-43-2 Tyrosine Kinase/Adaptors c-Kit inhibit SCFR CD117 Inhibitor ISCK-03 ISCK 03 inhibitor

 

陶术
生物
TargetMol®中国区唯一合作伙伴
点击进入陶术生物官网陶术生物
联系我们
400-820-0310

上海市静安区江场三路238号8楼