Powder: -20°C for 3 years | In solvent: -80°C for 1 year
INH1 (IBT13131) 通过直接结合Hec1,特异性干扰 Hec1/Nek2 的相互作用。它在体内外均表现出良好的抗肿瘤活性。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 118 | 现货 | ||
5 mg | ¥ 247 | 现货 | ||
10 mg | ¥ 413 | 现货 | ||
25 mg | ¥ 849 | 现货 | ||
50 mg | ¥ 1,490 | 现货 | ||
100 mg | ¥ 2,390 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 272 | 现货 |
产品描述 | INH1 (IBT13131) is a cell-permeable Hec1 inhibitor that specifically disrupts the Hec1/Nek2 interaction. |
靶点活性 | Hec1:10-21 μM |
体外活性 | 腹腔注射100 mg/kgINH1抑制含有MDA-MB-468人乳腺癌异种移植物的小鼠中乳腺肿瘤生长. |
体内活性 | INH1有效抑制人乳腺癌细胞增殖,GI50为10-21 μM。此外,INH1也会通过损害纺锤体监测点调控的Hec1/Nek2通路,引发细胞杀伤活性。 |
激酶实验 | Binding assays: Surface plasma resonance (SPR) assays are performed at 22.5°C in HBSD buffer [10 mmol/L HEPES, 150 mmol/L NaCl, 0.1% DMSO (pH 7.5)] on Biacore 3000. 6×His-Hec1 and GST-Nek2 are purified. NTA sensor chip or glutathione-modified CM5 chip are used to capture His-Hec1 and GST-Nek2, respectively. The capture level is about 140 to 180 resonance units (RU) at the flow rate of 5 μL/min. For the binding assay, chips are sequentially treated with compounds (1 or 20 μmol/L) and then proteins (50 μg/mL). Retained RUs are recorded and processed (triplicate experiments). |
细胞实验 | Standard 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays with a 3-d drug treatment procedure are performed to measure the dose-dependent cytotoxicity of INH1 in cultured cells. Triplicate sets are measured and compiled for final data presentation.(Only for Reference) |
别名 | IBT13131 |
分子量 | 308.4 |
分子式 | C18H16N2OS |
CAS No. | 313553-47-8 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 30.8 mg/mL (100 mM)
Ethanol: 3.1 mg/mL (10 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO / Ethanol | 1 mM | 3.2425 mL | 16.2127 mL | 32.4254 mL | 81.0636 mL |
5 mM | 0.6485 mL | 3.2425 mL | 6.4851 mL | 16.2127 mL | |
10 mM | 0.3243 mL | 1.6213 mL | 3.2425 mL | 8.1064 mL | |
DMSO | 20 mM | 0.1621 mL | 0.8106 mL | 1.6213 mL | 4.0532 mL |
50 mM | 0.0649 mL | 0.3243 mL | 0.6485 mL | 1.6213 mL | |
100 mM | 0.0324 mL | 0.1621 mL | 0.3243 mL | 0.8106 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
INH1 313553-47-8 Apoptosis Cytoskeletal Signaling Microtubule Associated inhibit INH 1 Inhibitor IBT-13131 INH-1 IBT13131 IBT 13131 inhibitor