Powder: -20°C for 3 years | In solvent: -80°C for 1 year
I-191 是选择性有效的蛋白酶激活受体 2 拮抗剂。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 1,170 | 现货 | ||
2 mg | ¥ 1,730 | 现货 | ||
5 mg | ¥ 2,930 | 现货 | ||
10 mg | ¥ 4,330 | 现货 | ||
25 mg | ¥ 6,920 | 现货 | ||
50 mg | ¥ 9,350 | 现货 | ||
100 mg | ¥ 12,600 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 2,990 | 现货 |
产品描述 | I-191 is a potent antagonist of protease-activated receptor 2 (PAR2), inhibits multiple signaling functions in human cancer cells. |
体外活性 | At nanomolar concentrations, I-191 inhibited PAR2 binding of and activation by structurally distinct PAR2 agonists (trypsin, peptide, nonpeptide) in a concentration-dependent manner in cells of the human colon adenocarcinoma grade II cell line (HT29).?I-191 potently attenuated multiple PAR2-mediated intracellular signaling pathways leading to Ca2+ release, extracellular signal-regulated kinase 1/2 (ERK1/2) phosphorylation, Ras homologue gene family, member A (RhoA) activation, and inhibition of forskolin-induced cAMP accumulation.?The mechanism of action of I-191 was investigated using binding and calcium mobilization studies in HT29 cells where I-191 was shown to be noncompetitive and a negative allosteric modulator of the agonist 2f-LIGRL-NH2 The compound alone did not activate these PAR2-mediated pathways, even at high micromolar concentrations, indicating no bias in these signaling properties.?I-191 also potently inhibited PAR2-mediated downstream functional responses, including expression and secretion of inflammatory cytokines and cell apoptosis and migration, in human colon adenocarcinoma grade II cell line (HT29) and human breast adenocarcinoma cells (MDA-MB-231).?I-191 is a potent PAR2 antagonist that inhibits multiple PAR2-induced signaling pathways and functional responses.?I-191 may be a valuable tool for characterizing PAR2 functions in cancer and in other cellular, physiological, and disease settings. |
分子量 | 423.48 |
分子式 | C23H26FN5O2 |
CAS No. | 1690172-25-8 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 8.33 mg/mL (19.67 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.3614 mL | 11.8069 mL | 23.6139 mL | 59.0347 mL |
5 mM | 0.4723 mL | 2.3614 mL | 4.7228 mL | 11.8069 mL | |
10 mM | 0.2361 mL | 1.1807 mL | 2.3614 mL | 5.9035 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
I-191 1690172-25-8 GPCR/G Protein Protease-activated Receptor Inhibitor I191 inhibit I 191 Protease Activated Receptor (PAR) Thrombin receptors inhibitor