Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Hesperidin methylchalcone 抑制氧化应激,细胞因子产生和NF-κB 活化。它能够抑制炎症和疼痛,具有血管保护作用。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 mg | ¥ 197 | 5日内发货 | ||
1 mL * 10 mM (in DMSO) | ¥ 460 | 5日内发货 |
产品描述 | Hesperidin methylchalcone is the Citrus original products with powerful antioxidant activity. It can suppresse experimental gout arthritis in mice by inhibiting NF-κB activation. |
体内活性 | Hesperidin methylchalcone (HMC) in a mouse model of gout arthritis induced by intra-articular injection of MSU (100 μg/10 μL).?Orally given HMC (3-30 mg/kg, 100 μL) reduced in a dose-dependent manner the MSU-induced hyperalgesia (44%, p 0.05), edema (54%, p 0.05), and leukocyte infiltration (70%, p 0.05).?HMC (30 mg/kg) inhibited MSU-induced infiltration of LysM-eGFP+ cells (81%, p 0.05), synovitis (76%, p 0.05), and oxidative stress (increased GSH, FRAP, and ABTS by 62, 78, and 73%, respectively;?reduced O2- and NO by 89 and 48%, p 0.05) and modulated cytokine production (reduced IL-1β, TNF-α, IL-6, and IL-10 by 35, 72, 37, and 46%, respectively, and increased TGF-β by 90%, p 0.05).?HMC also inhibited MSU-induced NF-κB activation (41%, p 0.05), gp91phox (66%, p 0.05) and NLRP3 inflammasome components mRNA expression in vivo (72, 77, 71, and 73% for NLRP3, ASC, pro-caspase-1, and pro-IL-1 β, respectively, p 0.05), and induced Nrf2/HO-1 mRNA expression (3.9- and 5.1-fold increase, respectively, p 0.05).?HMC (30, 100, and 300 μM) did not inhibit IL-1β secretion by macrophages primed by LPS and challenged with MSU (450 μg/mL), demonstrating that the anti-inflammatory effect of HMC in gout arthritis depends on inhibiting NF-κB but not on direct inhibition of inflammasome[1]. |
分子量 | 624.59 |
分子式 | C29H36O15 |
CAS No. | 24292-52-2 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 10 mg/mL (16.1 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 1.6011 mL | 8.0053 mL | 16.0105 mL | 40.0263 mL |
5 mM | 0.3202 mL | 1.6011 mL | 3.2021 mL | 8.0053 mL | |
10 mM | 0.1601 mL | 0.8005 mL | 1.6011 mL | 4.0026 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Hesperidin methylchalcone 24292-52-2 NF-Κb NF-κB Inhibitor Nuclear factor-kappaB inhibit Nuclear factor-κB inhibitor