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HAMNO

HAMNO

产品编号 T4311   CAS 138736-73-9
别名: NSC111847

HAMNO (NSC-111847) 是一种复制蛋白 A 的新型蛋白质相互作用抑制剂。

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HAMNO Chemical Structure
HAMNO, CAS 138736-73-9
规格 价格/CNY 货期 数量
1 mg ¥ 186 现货
5 mg ¥ 413 现货
10 mg ¥ 662 现货
25 mg ¥ 1,320 现货
50 mg ¥ 2,120 现货
100 mg ¥ 3,190 现货
1 mL * 10 mM (in DMSO) ¥ 455 现货
产品目录号及名称: HAMNO (T4311)
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生物活性
化学信息
存储 & 溶解度
参考文献
产品描述 HAMNO (NSC-111847) is a protein interaction inhibitor of replication protein A (RPA).
靶点活性 RPA:
体外活性 HAMNO inhibits colony formation in both HNSCC cell lines. The combination of HAMNO with etoposide markedly inhibits colony formation to a greater degree than HAMNO alone. In UMSCC38 cells, HAMNO dose-dependently increased the occurs of pan-nuclear γ-H2AX staining. UMSCC38 and UMSCC11B cells have prominent γ-H2AX staining, particularly after incubation with HAMNO (20 μM). In UMSCC38 and OKF4 cells, HAMNO increased γ-H2AX staining, with the greatest increase in signal occurring in S-phase.
体内活性 In mice, HAMNO inhibits the progression of UMSCC11B tumors. After treatment with etoposide (20 μM, 2 h), Ser33 of RPA32 (an ATR substrate) is highly phosphorylated, which is reduced with the addition of 2 μM HAMNO and is nearly absent at higher concentrations, demonstrating HAMNO can inhibit RPA32 phosphorylation by ATR.
细胞实验 Cell cycle assessment and γ-H2AX staining are monitored in UMSCC38 and OKF4 cells after 2 h incubation with HAMNO (2, 20, 50 μM) and fixed in 70% ethanol overnight. Cells are washed with PBS and incubated overnight in PBS containing 1% BSA, 10% goat serum, and PS139-H2AX antibodies washed and incubated in goat anti-mouse Alexa Fluor 647 antibody for 30 min at room temperature. Cells are incubated in 50 μg/mL propidium iodide and 100 μg/mL RNase A for 30 min, and 10,000 cells per sample are analyzed.
动物实验 UMSCC38 and UMSCC11B cells are implanted into 6-week-old female mice by a single subcutaneous injection of tumor cells (2 to 6×105 cells in 100 mL of sterile PBS). The growth rates of tumors are determined by daily monitoring of tumor volume with vernier calipers [tumor volume=1/2(length×width2)]. Once the tumor size reaches 50 mm3, etoposide (10 mg/kg mouse) and HAMNO (2 mg/kg) are administered intraperitoneally every day for 3 days. Tumor size is monitored daily and the volume of the tumor is compared among all experimental groups.
别名 NSC111847
分子量 263.29
分子式 C17H13NO2
CAS No. 138736-73-9

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

DMSO: 155 mg/mL

计算器

摩尔浓度计算器
稀释计算器
配液计算器
分子量计算器
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输入分子式,点击计算,可计算出产品的分子量。

参考文献

1. Glanzer JG, et al. RPA inhibition increases replication stress and suppresses tumor growth. Cancer Res. 2014 Sep 15;74(18):5165-72.
Antiviral agent 36 Streptozocin DNA polymerase-IN-2 MM41 Setrobuvir DNA polymerase-IN-3 Vidofludimus Pritelivir

相关化合物库

该产品包含在如下化合物库中:
经典已知活性库 抗衰老化合物库 表型筛选靶点鉴定库 抑制剂库 细胞周期化合物库 PPI抑制剂库 NO PAINS 化合物库 已知活性化合物库 DNA 损伤和修复分子库 抗癌化合物库

剂量换算

对于不同动物的给药剂量换算,您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。

体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。

第一步:请输入动物实验的基本信息
剂量
mg/kg
每只动物体重
g
给药体积
μL
动物数量
第二步:请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
% DMSO
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% Tween 80
% ddH2O
计算 重置

技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

HAMNO 138736-73-9 Cell Cycle/Checkpoint DNA Damage/DNA Repair DNA/RNA Synthesis inhibit NSC111847 Inhibitor NSC 111847 NSC-111847 inhibitor

 

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