Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Guanidine hydrochloride (Aminoformamidine Hydrochloride) 是一种强离液剂和一种蛋白质的强变性剂。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
10 g | ¥ 99 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 228 | 现货 |
产品描述 | Guanidine hydrochloride (Aminoformamidine Hydrochloride) is a strong organic base existing primarily as guanidium ions at physiological pH. It is found in the urine as a normal product of protein metabolism. It is also used in laboratory research as a protein denaturant. |
体外活性 | Guanidine HCl is the most popular protein denaturant. Analysis of unfolding transitions by Guanidine HCl provides several important parameters regarding the mechanism of conformational stability of proteins. [1] Guanidine HCl at low concentrations refolds acid-unfolds apomyoglobin and cytochrome c, stabilizing the molten globule state. Guanidine HCl (> 1 M) causes co-operative unfolding of the molten globule state. [2] Guanidine HCl is 2.8 times more effective than urea in unfolding ribonuclease but only 1.7 times more effective for lysozyme. △GWaterapp. values of Guanidine HCl are 9.7 Cal/mole for ribonuclease at pH 6.6, 6.1 for lysozyme at pH 2.9, 8.3 for α-chymotrypsin at pH 4.3, and 11.7 for β-lactoglobulin at pH 3.2. [3] Guanidine HCl at millimolar concentrations, is able to causes efficient loss of the normally stable [PSI+] element from yeast cells. 5 mM Guanidine HCl in growth media cures [PSI+] and other prions of yeast. [4] 5 mM Guanidine HCl significantly reduces Hsp104-mediated basal and acquired thermotolerance by 30-fold and 50 fold, respectively. Guanidine HCl also reduces the ability of Hsp104 to restore activity of thermally denatured luciferase. [5] |
别名 | Guanidine HCl, Aminoformamidine HCl, 盐酸胍, Aminoformamidine Hydrochloride, Guanidinium chloride |
分子量 | 95.53 |
分子式 | CH6ClN3 |
CAS No. | 50-01-1 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 198.9 mM
H2O: 198.9 mM
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO / H2O | 1 mM | 10.4679 mL | 52.3396 mL | 104.6792 mL | 261.6979 mL |
5 mM | 2.0936 mL | 10.4679 mL | 20.9358 mL | 52.3396 mL | |
10 mM | 1.0468 mL | 5.234 mL | 10.4679 mL | 26.1698 mL | |
20 mM | 0.5234 mL | 2.617 mL | 5.234 mL | 13.0849 mL | |
50 mM | 0.2094 mL | 1.0468 mL | 2.0936 mL | 5.234 mL | |
100 mM | 0.1047 mL | 0.5234 mL | 1.0468 mL | 2.617 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Guanidine hydrochloride 50-01-1 Autophagy Cell Cycle/Checkpoint DNA Damage/DNA Repair Immunology/Inflammation Metabolism Others Arginase DNA/RNA Synthesis Endogenous Metabolite unfolded Guanidinium Chloride Hsp104 chaotrope Guanidine HCl denaturant Aminoformamidine HCl Guanidinium Guanidine Aminoformamidine Inhibitor Guanidine Hydrochloride 盐酸胍 Aminoformamidine Hydrochloride Guanidinium chloride inhibit proteins inhibitor