Powder: -20°C for 3 years | In solvent: -80°C for 1 year
GSK137647A (GSK 137647) 是一种选择性FFA4激动剂,作用于人类和大小鼠FFA4的pEC50分别为6.3、6.1和6.2。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 186 | 现货 | ||
2 mg | ¥ 258 | 现货 | ||
5 mg | ¥ 413 | 现货 | ||
10 mg | ¥ 662 | 现货 | ||
25 mg | ¥ 1,230 | 现货 | ||
50 mg | ¥ 1,990 | 现货 | ||
100 mg | ¥ 3,190 | 现货 | ||
200 mg | ¥ 4,570 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 455 | 现货 |
产品描述 | GSK137647A (GSK 137647) is a selective FFA4 agonist. |
激酶实验 | HDAC-inhibitory activity: For the enzyme assay, 10 μL of [3H]acetyl-labeled histones (25,000 cpm/10 μg) are added to 90 μL of the HDAC enzyme fraction extracted from 293T cells overexpressing HDAC1 or HDAC2 in the presence of increasing concentrations of Romidepsin, and the mixture is incubated at 37 °C for 15 minutes. The enzyme reaction is linear for at least 1 hour. The reaction is stopped by the addition of 10 μL of concentrated HCl. The released [3H]acetic acid is extracted with 1 mL of ethylacetate, and 0.9 mL of the solvent layer is taken into 5 mL of aqueous counting scintillant II solution for determination of radioactivity. The IC50 values are determined from at least three independent dose-response curves. |
别名 | GSK 137647 |
分子量 | 305.39 |
分子式 | C16H19NO3S |
CAS No. | 349085-82-1 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 50 mg/mL (163.73 mM)
H2O: < 0.06 mg/mL (insoluble or slightly soluble)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.2745 mL | 16.3725 mL | 32.745 mL | 81.8625 mL |
5 mM | 0.6549 mL | 3.2745 mL | 6.549 mL | 16.3725 mL | |
10 mM | 0.3275 mL | 1.6373 mL | 3.2745 mL | 8.1863 mL | |
20 mM | 0.1637 mL | 0.8186 mL | 1.6373 mL | 4.0931 mL | |
50 mM | 0.0655 mL | 0.3275 mL | 0.6549 mL | 1.6373 mL | |
100 mM | 0.0327 mL | 0.1637 mL | 0.3275 mL | 0.8186 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
GSK137647A 349085-82-1 Endocrinology/Hormones GPCR/G Protein GPR Free Fatty Acid Receptor FFA1 FFAR insulin secretion FFA3 GSK-137647 FFA4 inhibit GSK-137647A Inhibitor GSK137647 anti-inflammatory epithelial ion transport GSK 137647 FFA2 inhibitor