Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Furazolidone (Furoxone) 是一种具有抗菌和抗原虫活性的硝基呋喃衍生物,抑制AML1-ETO 转化细胞,IC50为12.7 μM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 mg | ¥ 415 | 现货 | ||
500 mg | ¥ 591 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 457 | 现货 |
产品描述 | Furazolidone (Furoxone), a nitrofuran derivative, inhibits AML1-ETO transformed cells with IC50 value of 12.7 μM. It is antibacterial and antiprotozoal activity, |
靶点活性 | AML1-ETO:12.7 μM |
体外活性 | Furazolidone displays potent antiproliferative properties at submicromolar concentrations and induces apoptosis in AML cell lines. Furazolidone treatment of certain AML cells induces myeloid cell differentiation by morphology and flow cytometry for CD11b expression, resulting in increased stability of tumor suppressor p53 protein in AML cells[1]. |
体内活性 | FZ accelerates its own metabolism in the chicken by induction of the activity of CPR whereas no effect is observed in the rat[2]. |
细胞实验 | Leukemic cells are seeded in 96-well culture plates at a density of 1 or 2×104 viable cells/100 µl/well in triplicates and are treated for 24, 48, and 72 hours with an incremental concentration of FZD ranging from 1 µM to 50 µM. Colorimetric CellTiter 96® Aqueous One Solution Cell Proliferation assay is used to determine the cytotoxicity. The optical density at 492 nm is measured using a Multiskan Ascent® microplate photometer. IC50 values are determined by MTS assay when cells are treated with FZD for 72 hours and calculated with GraphPad Prism 5. Each experiment was in triplicate. (Only for Reference) |
别名 | 呋喃唑酮, Furoxone |
分子量 | 225.16 |
分子式 | C8H7N3O5 |
CAS No. | 67-45-8 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 7.5 mg/mL (33.31 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 4.4413 mL | 22.2064 mL | 44.4129 mL | 111.0322 mL |
5 mM | 0.8883 mL | 4.4413 mL | 8.8826 mL | 22.2064 mL | |
10 mM | 0.4441 mL | 2.2206 mL | 4.4413 mL | 11.1032 mL | |
20 mM | 0.2221 mL | 1.1103 mL | 2.2206 mL | 5.5516 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Furazolidone 67-45-8 Apoptosis Metabolism Microbiology/Virology Neuroscience MAO Antibacterial Antibiotic Inhibitor 呋喃唑酮 Bacterial inhibit Furoxone inhibitor