Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Formosanin C (Polyphyllin B) 是从滇重楼中分离的一种薯蓣皂苷,可诱导细胞凋亡。它是一种免疫调节剂,具有抗肿瘤活性。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 638 | 现货 | ||
5 mg | ¥ 1,550 | 现货 | ||
10 mg | ¥ 2,770 | 现货 | ||
25 mg | ¥ 4,620 | 现货 | ||
50 mg | ¥ 6,590 | 现货 | ||
100 mg | ¥ 8,980 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 2,910 | 现货 |
产品描述 | Formosanin C (Polyphyllin B) is a compound isolated from Rhizoma Paridis, showed pro-apoptosis and immunoregulation with antitumor activity in cultured cells and animal systems. |
体外活性 | Attenuation of formosanin C-induced change of Deltapsi(m) by caspase-2 inhibitor (Z-VDVAC) implies that caspase-2 acts upstream of the mitochondria. Blockage of formosanin C-induced apoptotic process by using either permeability transition pore inhibitor (cyclosporine A) or caspase-9 inhibitor (Z-LEHD) demonstrates the necessity of mitochondria and caspase-9 in formosanin C-induced apoptosis of HT-29 cells. |
激酶实验 | The enzyme activity and kinetics of the purified ROCK1(3-543) are determined using scintillation proximity assay. In this assay, purified ROCK1 is incubated with peptide substrate (Biotin-Ahx-AKRRLSSLRA-CONH2), and 33ATP and the subsequent incorporation of 33P into the peptide is quantified by streptavidin bead capture. For IC50 determination, test compounds are dissolved at 10 mM in 100% DMSO, with subsequent serial dilution in 100% DMSO. Compounds are typically assayed over an 11-point dilution range with a concentration in the assay of 10 μM to 0.2 nM in 3-fold dilutions. For dose-response curves, data are normalized and expressed as percentage inhibition using the formula 100×[(U-C1)/(C2-C1)], where U is the unknown value, C1 is the average of the high signal (0%) control wells, and C2 is the average of the low signal (100%) control wells. Curve fitting is performed The results for each compound are recorded as pIC50 values[1]. |
别名 | Polyphyllin B, 重楼皂苷 B, 重楼皂苷B |
分子量 | 1015.18 |
分子式 | C51H82O20 |
CAS No. | 50773-42-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 27.5 mg/mL (27.09 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 0.985 mL | 4.9252 mL | 9.8505 mL | 24.6262 mL |
5 mM | 0.197 mL | 0.985 mL | 1.9701 mL | 4.9252 mL | |
10 mM | 0.0985 mL | 0.4925 mL | 0.985 mL | 2.4626 mL | |
20 mM | 0.0493 mL | 0.2463 mL | 0.4925 mL | 1.2313 mL |
中药材名称 | 中药材拉丁名 | 性 | 味 | 归经 |
七叶一枝花 | Paris polyphylla Smith var. chinensis (Franch.) Hara, P. polyphylla Smith | 寒 | 苦 | 心, 肝, 肺 |
中成药名称 | 处方组成 | 主治疾病 | 中成药类型 |
罗浮山百草油 | 两面针,徐长卿,九里香,辛夷花,红花,水芙蓉,还魂草,三七,千里光,大头陈,当归,鹅不食草,三七,肿节风,鸡骨香,砂仁,独活,羌活,生姜,陈皮,香附,野菊花,山白芷,桂枝,小罗伞,蔓荆子,桔梗,紫珠叶,地胆草,细辛,五指柑,肉豆蔻,木防己,三叉苦,金银花,救必应,白半枫荷,山苍子,麻黄,地念,防风,半枝莲,铁包金,柴胡,飞天禽劳,鸡骨草,荆芥,虎杖,钩藤,一枝黄花,白花灯笼,白花蛇舌草,人字草,金线风,石仙桃,五月艾,皂角刺,木香,山芝麻,益母草,紫苏叶,倒扣草,侧柏叶,金耳环,一朵云,七叶一枝花,鱼腥草 | 外用,涂搽患处。 | 解表药 |
季德胜蛇药片 | 七叶一枝花,蟾蜍,蜈蚣,地锦草 | 口服。一次1袋,一日3次,或遵医嘱。 | 清热药 |
方剂名称 | 处方组成 | 剂型 | 处方来源 |
梦灵丸 | 羊肝,七叶一枝花,玄精石,石决明,黄连,蕤核 | 丸剂 | 《圣济总录》卷一○二。 |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Formosanin C 50773-42-7 Apoptosis Proteases/Proteasome Caspase Polyphyllin B 重楼皂苷 B inhibit Inhibitor 重楼皂苷B inhibitor