keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Fluo-8,AM is one of the most commonly used fluorescent probes to detect intracellular calcium concentration.Fluo-8, which penetrates the cell membrane and enters the cell, is cut by the intracellular esterase to form fluO-8, and thus is trapped in the cell. Fluo-8 is almost non-fluorescent when it exists in the form of free ligand, but when it combines with intracellular calcium ions, it can produce strong fluorescence, with the maximum excitation wavelength of 490nm and the maximum emission wavelength of 514nM.
产品描述 | Fluo-8,AM is one of the most commonly used fluorescent probes to detect intracellular calcium concentration.Fluo-8, which penetrates the cell membrane and enters the cell, is cut by the intracellular esterase to form fluO-8, and thus is trapped in the cell. Fluo-8 is almost non-fluorescent when it exists in the form of free ligand, but when it combines with intracellular calcium ions, it can produce strong fluorescence, with the maximum excitation wavelength of 490nm and the maximum emission wavelength of 514nM. |
体外活性 | Load Cells with Fluo-8? AM Esters: Prepare a 2 to 5 mM stock solution of Fluo-8? AM esters in high-quality, anhydrous DMSO. Prepare a working solution of 1 to 10 μM in Hanks and Hepes buffer (HHBS). For most of cell lines, Fluo-8? reagents with a concentration ranging from 4-5 uM are recommended. Probenecid (1–2.5 mM) or sulfinpyrazone (0.1–0.25 mM) may be added to the cell medium to reduce leakage of the de-esterified indicators. Add equal volume of the dye working solution into your cell plate. Incubate the dye-loading plate at a cell incubator (20-60minutes). Replace the dye working solution with HHBS to remove excess probes. Run the experiments at Ex/Em = 490/525 nm. |
分子量 | 1060.3 |
分子式 | C51H52N2O23 |
CAS No. | TD0055 |
keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Fluo-8, AM TD0055 Fluo 8, AM Fluo8, AM Inhibitor inhibitor inhibit