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Fluo-3, pentapotassium salt

Fluo-3, pentapotassium salt

产品编号 TD0053   CAS TD0053

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3 and Fluo-4 are most commonly used among the visible light-excitable calcium indicators. Fluo-4 is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels.

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Fluo-3, pentapotassium salt
Fluo-3, pentapotassium salt, CAS TD0053
规格 价格/CNY 货期 数量
1 mg ¥ 3,287 待询
其他形式的 Fluo-3, pentapotassium salt:
千万补贴 助力科研
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产品目录号及名称: Fluo-3, pentapotassium salt (TD0053)
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存储 & 溶解度
产品描述 Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3 and Fluo-4 are most commonly used among the visible light-excitable calcium indicators. Fluo-4 is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels.
体外活性 Prepare a 2 to 5 mM AM esters stock solution in high-quality, anhydrous DMSO. Prepare a working solution of 2 to 20 μM in the buffer. For most cell lines we recommend the final concentration of calcium indicators be 4-5 uM. Probenecid or sulfinpyrazone may be added to the the dye working solution (final in well concentration will be 1-2.5 mM for probenecid, or 0.1 -0.25 mM for sulfinpyrazone) to reduce the leakage of the deesterified indicators. Add equal volume of the dye working solution into your cell plate. Incubate the plate at room temperature (20-120 minutes), then incubate the plate at room temperature for another 30 minutes. Replace the dye working solution with HHBS to remove excess probes. Run the experiments at Ex= 506nm, Em=526nm.
分子量 959.99
分子式 C36H25Cl2K5N2O13
CAS No. TD0053

存储

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year

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Keywords

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