Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Flucytosine (NSC 103805) 是抗真菌化合物。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
500 mg | ¥ 291 | 现货 | ||
1 g | ¥ 375 | 现货 | ||
5 g | ¥ 865 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 418 | 现货 |
产品描述 | Flucytosine (NSC 103805) is a pyrimidine compound and a fluorinated cytosine analog exhibiting antifungal activity. |
体外活性 | 在感染新型隐球菌的A/JCr小鼠中,Flucytosine和单抗2H1联合处理效果比单独给药效果好,明显降低肺部菌落形成. |
体内活性 | Lucytosine(≥1.25 mg / L)抑制Sabouraud葡萄糖培养液中C. neoformans的生长。 |
激酶实验 | Microdilution method: The culture media used are RPMI 1640 with glutamine, without bicarbonate and phenol red, buffered with morpholinopropanesulfonic acid (MOPS) (0.165 M, pH 7.0). Two-fold serial dilutions of Flucytosine (0.06-64 μg/mL) are prepared and dispensed in 50 uL aliquot, in flat-bottom 96-well assay plates which are kept frozen at -70 °C in sealed plastic bags until used. The inoculum is prepared spectrophotometrically and standardized to a concentration of 1.0-5.0 × 103 cfu per mL. A 50 μL volume of this suspension is used to inoculate each well containing 50 μL of the double concentration of Flucytosine to be tested. Once inoculated, each well therefore contains 100 μL of broth favoured over 200 μL to facilitate the agitation of the plates prior to spectrophotometric reading. After an incubation period of 24 and 48 hours at 35 °C, the plates are agitated for 3 minutes at 900 r.p.m. with a shaker and the optical density of the growth in each well is determined with the use of an automatic plate reader set at 495 nm. The inhibitory concentration of IC50 is computed mathematically. |
细胞实验 | J774.16 murine macrophage-like cells plated on 96-well tissue culture plates are incubated with 500 u/mL murine recombinant gamma-interferon overnight at 37 °C. The medium is then replaced with fresh medium containing gamma-interferon 500 μ/mL, LPS 3 mg/L, C. neoformans cells 1.6 × 104/well and Flucytosine 50 mg/L. Plates are incubated for 24 hours at 37 °C. Cell supernatants are collected and cells lysed by adding 0.1 mL sterile distilled water to each well, incubating at room temperature for 30 minutes, and then aspirating and ejecting the lysate with a pipette several times to complete cell disruption. Wells are rinsed with PBS (0.1 mL) and the cell supernatant, and lysate and rinse from each well are pooled, vortexed, diluted 1:50, vortexed again and spread on Sabouraud's dextrose agar for the cfu determination(Only for Reference) |
别名 | 5-Fluorocytosine, Ro 2-9915, 5-氟胞嘧啶, Fluorocytosine, NSC 103805 |
分子量 | 129.09 |
分子式 | C4H4FN3O |
CAS No. | 2022-85-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 8 mg/mL (61.97 mM)
H2O: 5 mg/mL (38.73 mM)
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO / H2O | 1 mM | 7.7465 mL | 38.7327 mL | 77.4653 mL | 193.6633 mL |
5 mM | 1.5493 mL | 7.7465 mL | 15.4931 mL | 38.7327 mL | |
10 mM | 0.7747 mL | 3.8733 mL | 7.7465 mL | 19.3663 mL | |
20 mM | 0.3873 mL | 1.9366 mL | 3.8733 mL | 9.6832 mL | |
DMSO | 50 mM | 0.1549 mL | 0.7747 mL | 1.5493 mL | 3.8733 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Flucytosine 2022-85-7 Cell Cycle/Checkpoint DNA Damage/DNA Repair Microbiology/Virology DNA/RNA Synthesis Antibiotic Antifungal Fungal NSC103805 5-Fluorocytosine Inhibitor Ro 2-9915 5-氟胞嘧啶 Fluorocytosine NSC 103805 inhibit NSC-103805 inhibitor