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FCCP

FCCP

产品编号 T6834   CAS 370-86-5
别名: Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone, Trifluoromethoxy carbonylcyanide phenylhydrazone

FCCP (Trifluoromethoxy carbonylcyanide phenylhydrazone) 是一种线粒体中氧化磷酸化解偶联剂,能够诱导 PINK1 激活,促进 Parkin 在 Ser65 位点磷酸化。

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FCCP Chemical Structure
FCCP, CAS 370-86-5
规格 价格/CNY 货期 数量
5 mg ¥ 283 现货
10 mg ¥ 425 现货
25 mg ¥ 862 现货
50 mg ¥ 1,690 现货
100 mg ¥ 2,860 现货
200 mg ¥ 4,220 现货
500 mg ¥ 6,590 现货
1 mL * 10 mM (in DMSO) ¥ 298 现货
产品目录号及名称: FCCP (T6834)
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生物活性
化学信息
存储 & 溶解度
参考文献
产品描述 FCCP (Trifluoromethoxy carbonylcyanide phenylhydrazone), a potent uncoupler of oxidative phosphorylation in mitochondria, transport protons across cell membranes leading to disrupts ATP synthesis.
体外活性 FCCP treatment induces a very rapid 2-fold increase in intracellular Ca2+ concentration that is accompanied by a strong protein synthesis rate inhibition. The translation inhibition correlates with an increased phosphorylation of the α subunit of eIF2 (eIF2α) and a 1.7-fold increase in the double-stranded RNA-dependent protein kinase activity[1]. FCCP treatment also mildly decreases ATP and reactive oxygen species levels. It increases the expression of mitochondrial genes such as Tfam and COXIV while inducing morphological features of quiescent mouse HSCs and abrogating TGF-β signal transduction[2].
体内活性 FCCP significantly reduces mitochondrial membrane potential and ATP production in 8-cell mouse embryos and the number of inner cell mass cells within blastocysts with unchanged blastocyst development. This perturbed embryonic mitochondrial function is concomitant with reduced birth weight in female offspring following embryo transfer, which persists until weaning. Although FCCP-treated males also exhibits reduced glucose tolerance as female, but their insulin sensitivity and adiposity gain between 4 and 14 weeks is unchanged. Reducing mitochondrial function and, thus, decreasing ATP output in the precompacting embryo can influence offspring phenotype[3].
细胞实验 Protein synthesis rate is assayed in 24-mm diameter multi-well dishes with fresh medium containing 0.175 Ci/mmol of [3H]methionine (200 μM), for 30 min at 37°C. PC12 cells are treated with FCCP for different period of times. (Only for Reference)
别名 Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone, Trifluoromethoxy carbonylcyanide phenylhydrazone
分子量 254.17
分子式 C10H5F3N4O
CAS No. 370-86-5

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

DMSO: 25.4 mg/mL (100 mM)

溶液配制表

可选溶剂 浓度 体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.9344 mL 19.6719 mL 39.3437 mL 98.3594 mL
5 mM 0.7869 mL 3.9344 mL 7.8687 mL 19.6719 mL
10 mM 0.3934 mL 1.9672 mL 3.9344 mL 9.8359 mL
20 mM 0.1967 mL 0.9836 mL 1.9672 mL 4.918 mL
50 mM 0.0787 mL 0.3934 mL 0.7869 mL 1.9672 mL
100 mM 0.0393 mL 0.1967 mL 0.3934 mL 0.9836 mL

计算器

摩尔浓度计算器
稀释计算器
配液计算器
分子量计算器
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输入分子式,点击计算,可计算出产品的分子量。

参考文献

1. Muñoz F, et al. FEBS Lett. 2001, 492(1-2):156-159. 2. Guimarães EL, et al. BMC Gastroenterol. 2012, 12:68. 3. Zander-Fox DL, et al. Biol Reprod. 2015, 92(5):124. 4. Shi J, Zhang F, Chen L, et al. Systemic mitochondrial disruption is a key event in the toxicity of bacterial pore‐forming toxins to Caenorhabditis elegans[J]. Environmental Microbiology.

文献引用

1. Liu H, Sun Y, Xu H, et al. PTEN-induced putative kinase 1 regulates mitochondrial quality control and is essential for the maturation of human induced pluripotent stem cell-derived cardiomyocytes. Genes & Diseases. 2022 2. Liang S, Zhu C, Suo C, et al. Mitochondrion-Localized SND1 Promotes Mitophagy and Liver Cancer Progression Through PGAM5. Frontiers in Oncology. 2022, 12: 857968-857968 3. Shi J, Zhang F, Chen L, et al. Systemic mitochondrial disruption is a key event in the toxicity of bacterial pore‐forming toxins to Caenorhabditis elegans. Environmental Microbiology. 2021 Sep;23(9):4896-4907
IACS-010759 Lipoic acid UK-5099 IMT1 Mitochonic acid 5 Leramistat Mito-TEMPO Kresoxim-Methyl

相关化合物库

该产品包含在如下化合物库中:
抗癌化合物库 抗衰老化合物库 离子通道库 线粒体靶向库 代谢化合物库 表型筛选靶点鉴定库 细胞凋亡化合物库 经典已知活性库 含氟化合物库 铜死亡化合物库

剂量换算

对于不同动物的给药剂量换算,您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。

体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。

第一步:请输入动物实验的基本信息
剂量
mg/kg
每只动物体重
g
给药体积
μL
动物数量
第二步:请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
% DMSO
%
% Tween 80
% ddH2O
计算 重置

技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

FCCP 370-86-5 Membrane transporter/Ion channel Metabolism ATPase Mitochondrial Metabolism inhibit Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone Inhibitor Trifluoromethoxy carbonylcyanide phenylhydrazone inhibitor

 

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