Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Eupalinolide A 是分离于林泽兰中的一种天然产物,通过抑制 HSF1 与 HSP90 的相互作用,激活 HSF1,诱导 HSP70 的表达。它对 A-549、BGC-823、SMMC-7721 和 HL-60 肿瘤细胞系具有强大的细胞毒性。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 258 | 现货 | ||
5 mg | ¥ 588 | 现货 | ||
10 mg | ¥ 857 | 现货 | ||
25 mg | ¥ 1,430 | 现货 | ||
50 mg | ¥ 2,120 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 660 | 现货 |
产品描述 | Eupalinolide B is a natural product ,and demonstrates potent cytotoxicity against A-549, BGC-823, SMMC-7721, and HL-60 tumour cell lines. |
体外活性 | Eupalinolide B and A (EB and EA) from E. lindleyanum, and describe their actions as HSP-inducers.?EA and EB both induced the expression of HSP70 in cells at concentrations that did not significantly affect cell viability.?Treatment of cells with EA or EB activated heat shock factor 1 (HSF1), while the artificial suppression of HSF1 expression diminished the EA- or EB-mediated induction of HSP70 expression.?Furthermore, EB inhibited the interaction between HSF1 and HSP90, which is known to inhibit the activity of HSF1.?Suggest that EA and EB induce the expression of HSP70 via the activation of HSF1 by inhibiting the interaction between HSF1 and HSP90.?EA and EB both induced the expression of HSP70 synergistically with other stressors.?Furthermore, pre-treatment of cells with EA or EB suppressed melanin production and stressor-induced apoptosis.?These effects were suppressed by the artificial suppression of HSP70 expression.?In vivo, the percutaneous administration of EB induced the expression of HSP70 and suppressed UVB radiation-induced damage, inflammatory responses and melanin production in the skin.[1] |
分子量 | 462.49 |
分子式 | C24H30O9 |
CAS No. | 877822-40-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 50 mg/mL (108.11 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.1622 mL | 10.811 mL | 21.6221 mL | 54.0552 mL |
5 mM | 0.4324 mL | 2.1622 mL | 4.3244 mL | 10.811 mL | |
10 mM | 0.2162 mL | 1.0811 mL | 2.1622 mL | 5.4055 mL | |
20 mM | 0.1081 mL | 0.5406 mL | 1.0811 mL | 2.7028 mL | |
50 mM | 0.0432 mL | 0.2162 mL | 0.4324 mL | 1.0811 mL | |
100 mM | 0.0216 mL | 0.1081 mL | 0.2162 mL | 0.5406 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Eupalinolide A 877822-40-7 Cytoskeletal Signaling Metabolism HSP responses inflammatory HSF1 expression Inhibitor production UV-induced melanin skin damage Heat shock proteins inhibit inhibitor