Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Esomeprazole Magnesium (NEXIUM) 是一种口服有效的 H+, K+-ATPase 抑制剂,在上消化道疾病和胃食管反流疾病中具有研究价值。Esomeprazole magnesium 是一种外泌体抑制剂,通过抑制 V-H+-ATPases 来阻断外泌体的释放。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
25 mg | ¥ 287 | 现货 | ||
50 mg | ¥ 410 | 现货 | ||
100 mg | ¥ 565 | 现货 | ||
200 mg | ¥ 828 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 434 | 现货 |
产品描述 | Esomeprazole Magnesium (NEXIUM) is the S-isomer of omeprazole, can reduce gastric acid secretion with selective and irreversible proton pump inhibitor activity.Esomeprazole magnesium acts as an exosome inhibitor by blocking the exosome release via the inhibition of V-H+-ATPases |
激酶实验 | Purified enzyme assays: ATM for use in the in vitro assay is obtained from HeLa nuclear extract by immunoprecipitation with rabbit polyclonal antiserum raised to the COOH-terminal 400 amino acids of ATM in buffer containing 25 mM HEPES (pH 7.4), 2 mM MgCl2, 250 mM KCl, 500 μM EDTA, 100 μM Na3VO4, 10% v/v glycerol, and 0.1% v/v Igepal. ATM-antibody complexes are isolated from nuclear extract by incubating with protein A-Sepharose beads for 1 hour and then through centrifugation to recover the beads. In the well of a 96-well plate, ATM-containing Sepharose beads are incubated with 1 μg of substrate glutathione S-transferase–p53N66 (NH2-terminal 66 amino acids of p53 fused to glutathione S-transferase) in ATM assay buffer [25 mM HEPES (pH 7.4), 75 mM NaCl, 3 mM MgCl2, 2 mM MnCl2, 50 μM Na3VO4, 500 μM DTT, and 5% v/v glycerol] at 37 °C in the presence or absence of inhibitor. After 10 minutes with gentle shaking, ATP is added to a final concentration of 50 μM and the reaction continued at 37 °C for an additional 1 hour. The plate is centrifuged at 250 × g for 10 minutes (4 °C) to remove the ATM-containing beads, and the supernatant is removed and transferred to a white opaque 96-well plate and incubated at room temperature for 1.5 hours to allow glutathione S-transferase-p53N66 binding. This plate is then washed with PBS, blotted dry, and analyzed by a standard ELISA technique with a phospho-serine 15 p53 antibody. The detection of phosphorylated glutathione S-transferase-p53N66 substrate is performed in combination with a goat antimouse horseradish peroxidase-conjugated secondary antibody. Enhanced chemiluminescence solution is used to produce a signal and chemiluminescent detection is carried out. |
别名 | (S)-Omeprazole magnesium, NEXIUM, 埃索美拉唑镁, (-)-Omeprazole magnesium |
分子量 | 713.12 |
分子式 | C34H36MgN6O6S2 |
CAS No. | 161973-10-0 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: 143 mg/mL (200.52 mM)
H2O: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 143 mg/mL (200.52 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
Ethanol / DMSO | 1 mM | 1.4023 mL | 7.0114 mL | 14.0229 mL | 35.0572 mL |
5 mM | 0.2805 mL | 1.4023 mL | 2.8046 mL | 7.0114 mL | |
10 mM | 0.1402 mL | 0.7011 mL | 1.4023 mL | 3.5057 mL | |
20 mM | 0.0701 mL | 0.3506 mL | 0.7011 mL | 1.7529 mL | |
50 mM | 0.028 mL | 0.1402 mL | 0.2805 mL | 0.7011 mL | |
100 mM | 0.014 mL | 0.0701 mL | 0.1402 mL | 0.3506 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Esomeprazole Magnesium 161973-10-0 Membrane transporter/Ion channel ATPase Proton pump (S)-Omeprazole antioxidant inhibit K+-ATPase Esomeprazole Cu/Zn-superoxide disorders Omeprazole magnesium intestinal (S)-Omeprazole magnesium Proton Pump NEXIUM 埃索美拉唑镁 (-)-Omeprazole magnesium Inhibitor inhibitor