Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Epacadostat (INCB 024360) 是选择性的IDO1抑制剂,IC50=71.8 nM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
2 mg | ¥ 256 | 现货 | ||
5 mg | ¥ 412 | 现货 | ||
10 mg | ¥ 661 | 现货 | ||
25 mg | ¥ 1,230 | 现货 | ||
50 mg | ¥ 1,890 | 现货 | ||
100 mg | ¥ 2,990 | 现货 | ||
200 mg | ¥ 4,390 | 现货 | ||
500 mg | ¥ 6,900 | 现货 | ||
1 g | ¥ 9,490 | 现货 | ||
2 g | ¥ 12,800 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 453 | 现货 |
产品描述 | Epacadostat (INCB 024360) is an oral-available inhibitor of indoleamine 2, 3-dioxygenase (IDO1), increasing the proliferation and activation of various immune cells and reducing tumor-associated regulatory T cells. |
靶点活性 | IDO1:10 nM |
体外活性 | In IFN-γ–treated human HeLa cells, INCB024360 potently inhibits kynurenine production. INCB024360 also promotes T and natural killer (NK)-cell growth, increases IFN-gamma production, and reduces conversion to regulatory T (T(reg))-like cells. [1] |
体内活性 | INCB024360 (100 mg/kg, p.o.), via IDO1 inhibition, suppresses kyn generation and tumor growth in immunocompetent, but not immunodeficient, mice. [1] In mice bearing CT26 colon carcinoma, INCB024360 (100 mg/kg, p.o.) also inhibits the growth of IDO-expressing tumors by reducing kynurenine. [2] |
细胞实验 | INCB 024360 (INCB024360) is dissolved in DMSO and stored, and then diluted with appropriate media before use[1]. To determine INCB 024360 activity against IDO in recombinant cells, HEK293/MSR cells are transiently transfected with full-length human or mouse IDO1, or mouse IDO2 cDNA, with Transit-293 transfection reagent or Lipofectamine 2000 reagents. INCB 024360 at different concentrations is added to the recovered transfected cells seeded at 2×104 cells per well in a 96-well plate (200 μL/well). The cells are incubated for 2 days, and kyn in the supernatants is measured as described in the HeLa cell assay. The tryptophan 2,3-dioxygenase (TDO) assay is performed similarly with HEK293/MSR cells transfected with a human TDO expression vector[1]. |
别名 | INCB 024360, 艾卡哚司他, IDO Inhibitor 1 |
分子量 | 438.23 |
分子式 | C11H13BrFN7O4S |
CAS No. | 1204669-58-8 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: 53 mg/mL (120.94 mM)
DMSO: 79 mg/mL(180.8 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
Ethanol / DMSO | 1 mM | 2.2819 mL | 11.4095 mL | 22.8191 mL | 57.0477 mL |
5 mM | 0.4564 mL | 2.2819 mL | 4.5638 mL | 11.4095 mL | |
10 mM | 0.2282 mL | 1.141 mL | 2.2819 mL | 5.7048 mL | |
20 mM | 0.1141 mL | 0.5705 mL | 1.141 mL | 2.8524 mL | |
50 mM | 0.0456 mL | 0.2282 mL | 0.4564 mL | 1.141 mL | |
100 mM | 0.0228 mL | 0.1141 mL | 0.2282 mL | 0.5705 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Epacadostat 1204669-58-8 Metabolism IDO Indoleamine 2,3-Dioxygenase (IDO) inhibit INCB024360 INCB-024360 Inhibitor INCB 024360 艾卡哚司他 IDO Inhibitor 1 inhibitor