keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Endomorphin 2 是一种高亲和力和选择性的 μ-阿片受体激动剂,对 kappa3 结合位点具有合理的亲和力 ,Ki 为 20 到 30 nM 之间。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 382 | 现货 | ||
2 mg | ¥ 548 | 现货 | ||
5 mg | ¥ 725 | 现货 | ||
10 mg | ¥ 1,190 | 现货 | ||
25 mg | ¥ 2,580 | 现货 | ||
50 mg | ¥ 3,230 | 现货 | ||
100 mg | ¥ 4,730 | 现货 |
产品描述 | Endomorphins-2 (EM-2) could decrease both the frequency and amplitude of the sEPSC of the motoneurons in lamina IX, which was reversed by the MOR antagonist CTOP. EM-2-IR fibers originated from primary afferent fibers form symmetric synaptic connections with motoneurons innervating skeletal muscles of the lower limbs in lamina IX of the spinal ventral horn and EM-2 might exert inhibitory effects on the activities of these motoneurons through both presynaptic and postsynaptic mechanisms. |
靶点活性 | μ opioid receptor:20-30 nM(Ki) |
体内活性 | After surviving for 3 days, all the rats retrograde labeled by WGA-HRP were deeply anesthetized by injection of an overdose of sodium pentobarbital (100 mg/kg), and then perfused with 100 ml of 0.01 M PBS (pH 7.4), followed by 500 ml of a fixative consisting of 4% paraformaldehyde and 0.01% glutaraldehyde in 0.1 M PB (pH 7.4). The spinal cord were removed and stored in 0.1 M PB (pH 7.4) containing of 4% paraformaldehyde at 4°C for 2–4 h. The intact sections were chose for TMR and Endomorphin-2(EM-2) or MOR double staining. The sections were blocked with 10% normal goat serum in 0.01 M PBS (pH 7.4) and then incubated for 24 h with guinea pig anti-TMR (1:500) and rabbit anti-EM-2 (1:200) or rabbit anti-MOR (1:500) antibodies at room temperature (RT). After washing in 0.01 M PBS (pH 7.4), sections were incubated for 8 h at RT with: (1) biotinylated donkey anti-rabbit IgG (1:500) and Alexa594-conjugated donkey anti-guinea pig IgG (1:500) diluted with PBS-NDS (for TMR and EM-2 staining); (2) biotinylated donkey anti-rabbit IgG (1:500) and Alexa488-conjugated donkey anti-guinea pig IgG (1:500) diluted with PBS-NDS (for TMR and MOR staining). Then, after washing in 0.01 M PBS (pH 7.4), sections were incubated with FITC-Avidin (1:1,000) (for EM-2 staining) or Alexa594-Avidin (1:1,000) (for MOR staining) in 0.01 M PBS containing 0.5% Triton X-100 (PBS-X) for 3 h at RT. Finally, the sections were rinsed with 0.01 M PBS, mounted onto clean glass slides, air-dried and cover slipped with a mixture of 0.05 M PBS containing 50% (v/v) glycerin and 2.5% (w/v) triethylenediamine. Images were taken using a laser-scanning confocal microscope. |
别名 | 内吗啡肽 2 |
分子量 | 571.67 |
分子式 | C32H37N5O5 |
CAS No. | 141801-26-5 |
keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 10 mM
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 1.7493 mL | 8.7463 mL | 17.4926 mL | 43.7315 mL |
5 mM | 0.3499 mL | 1.7493 mL | 3.4985 mL | 8.7463 mL | |
10 mM | 0.1749 mL | 0.8746 mL | 1.7493 mL | 4.3732 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Endomorphin 2 141801-26-5 Endocrinology/Hormones GPCR/G Protein Neuroscience Opioid Receptor Endomorphin2 inhibit Inhibitor 内吗啡肽 2 Endomorphin-2 inhibitor