store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
EIPA (L593754) 是一种有效的TRPP3 channel 抑制剂,IC50为 10.5 μM。EIPA 对Na+/H+交换 (NHE) 和巨噬细胞也有抑制作用。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
2 mg | ¥ 258 | 现货 | ||
5 mg | 待询 | 现货 | ||
50 mg | 待询 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 455 | 现货 |
产品描述 | EIPA (L593754) (L593754, MH 12-43) is a TRPP3 channel inhibitor (IC50: 10.5 μM). It also inhibits Na+/H+-exchanger (NHE) and macropinocytosis. |
靶点活性 | TRPP3 channel:10.5 μM |
体外活性 | It is found that EIPA, benzamil, and phenamil rapidly and reversibly block Ca2+-activated TRPP3 channel activation at -50 mV, with IC50s of 143, 10.5, 1.1, and 0.14 μM, respectively[1]. The number of autophagic vacuoles increases dramatically in the HAE and HPE groups after EIPA treatment compares with the HAN and HPN groups. EIPA regulates the initiation and maturation of the autophagy associated with amino acids in IEC-18 cells [2]. In addition, the uptake of cinnamoylphenazine (CA-PZ) and neutral red (NR) is inhibited by EIPA [3]. |
细胞实验 | The effect of EIPA alone (without alanine or proline) is also examined in both control (DMEM cultured cells) and amino acid-starved cells. The cells are incubated for 6 h in DMEM containing 5% FBS and either 0 or 0.3 mM EIPA (labeled QNN and QNE, respectively), HBSS containing either 0 or 0.3 mM EIPA (labeled HNN and HNE, respectively), HBSS with 1.0 mM alanine (labeled HAN) or 0.5 mM proline (labeled HPN), HBSS with 1.0 mM alanine and 0.3 mM EIPA (labeled HAE), and HBSS with 0.5 mM proline and 0.3 mM EIPA (labeled HPE) [2]. |
别名 | L593754, MH 12-43 |
分子量 | 299.76 |
分子式 | C11H18ClN7O |
CAS No. | 1154-25-2 |
store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 135 mg/mL (450.36 mM), sonification is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.336 mL | 16.68 mL | 33.36 mL | 83.4001 mL |
5 mM | 0.6672 mL | 3.336 mL | 6.672 mL | 16.68 mL | |
10 mM | 0.3336 mL | 1.668 mL | 3.336 mL | 8.34 mL | |
20 mM | 0.1668 mL | 0.834 mL | 1.668 mL | 4.17 mL | |
50 mM | 0.0667 mL | 0.3336 mL | 0.6672 mL | 1.668 mL | |
100 mM | 0.0334 mL | 0.1668 mL | 0.3336 mL | 0.834 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
EIPA 1154-25-2 Membrane transporter/Ion channel Sodium Channel TRP/TRPV Channel colon carcinoma IEC-18 NHE3 renal failure IBDs Cation channel ddY strain mice MKN28 L-593754 Autophagy gastric cancer Inhibitor neuron pancreatic carcinoma inhibit L593754 IECs TRP Channel L 593754 Sodium-hydrogen Exchanger Prostaglandin Receptor Cyclooxygenase COX Na+/H+ Exchanger (NHE) zinc Transient receptor potential channels calcium kainite MH 12-43 Sodium/hydrogen Exchanger inhibitor