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Diquafosol tetrasodium

Diquafosol tetrasodium

产品编号 T7423   CAS 211427-08-6
别名: 地夸磷索四钠, INS365

Diquafosol tetrasodium (INS365) 是一种 P2Y2 受体激动剂,可刺激眼组织粘蛋白的分泌。它可改善泪膜稳定性和视觉功能,作为干眼症的局部治疗。

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Diquafosol tetrasodium Chemical Structure
Diquafosol tetrasodium, CAS 211427-08-6
规格 价格/CNY 货期 数量
1 mg ¥ 256 现货
2 mg ¥ 365 现货
5 mg ¥ 592 现货
10 mg ¥ 957 现货
25 mg ¥ 1,750 现货
50 mg ¥ 2,930 现货
100 mg ¥ 4,330 现货
500 mg ¥ 9,370 现货
其他形式的 Diquafosol tetrasodium:
产品目录号及名称: Diquafosol tetrasodium (T7423)
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纯度: 99.68%
纯度: 98.7%
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生物活性
化学信息
存储 & 溶解度
参考文献
产品描述 Diquafosol Tetrasodium is a P2Y2 receptor agonist that stimulates the secretion of mucins from ocular tissues Diquafosol tetrasodium (INS365) improves tear film stability and visual function, as a topical treatment of dry eye disease.
体外活性 duration of exposure and diquafosol concentration significantly reduced cell survival and increased cell lysis.?On the other hand, no significant difference was observed in HA groups regardless of exposure time.?Cell viability significantly decreased after treatment for 1 hour with 30% diluted diquafosol and after treatment for 6 hours with all diluted diquafosol.?After treatment with all diluted diquafosol for 24 hours, cell viability decreased and lysis increased, and significant cellular damage was observed[1].
体内活性 In a rat dry eye model, the P2Y(2) agonist INS365 was found to improve surface health, based on increases in tear fluid secretion, corneal epithelial resistance, and release of glycoprotein-containing moieties from goblet cells.??INS365 is a potential therapeutic agent for use in the treatment of dry eye syndrome[2].
细胞实验 The viabilities of human corneal epithelial cells (HCECs) were determined using a MTT assay.?Cells (100 μl;?5×104 cell/mL) were plated in 96-well tissue-culture plates and incubated at 37℃ in 5% CO2 for 24 to 48 h until cultures were subconfluent.?Diquafosol (100 μl diluted 10%, 20%, or 30%) or 0.3% or 0.18% HA were added and incubated for 1, 6, or 24 h. DMEM (100 μl) was added to controls.?After 1, 6, and 24 h, plates were washed three times with PBS to remove the drugs.?Cell viabilities were evaluated after incubating for 24 h. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide;?thiazoyl blue (5 mg/mL) was then added to each well.?Samples were incubated in the dark for 4 h at 37℃, and media were then removed.?Precipitates were resuspended indimethyl sulfoxide (100 μl;?DMSO, Sigma-Aldrich).?Absorbances were measured on a plate reader at 570 nm.?The experiment was performed in triplicate[1].
动物实验 An SD rat dry eye model was used in which exorbital lacrimal gland extirpation decreased the Schirmer test score by at least 50%. After 8 weeks, when significant increases occurred in corneal epithelial permeability, Diquafosol tetrasodium(INS365)-containing eye drops were applied six times daily for the next 4 weeks at concentrations from 0.03% to 3.0%. Corneal barrier function was evaluated based on measurements with a modified anterior fluorometer of fluorescein penetrance at 1, 2, and 4 weeks after initial application. After INS365 application, the periodic acid-Schiff reagent (PAS)-stained area was evaluated in histologic sections of the tarsal and bulbar conjunctiva[2].
别名 地夸磷索四钠, INS365
分子量 878.23
分子式 C18H22N4Na4O23P4
CAS No. 211427-08-6

存储

keep away from direct sunlight,store under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

H2O: 10 mM

DMSO: Insoluble

溶液配制表

可选溶剂 浓度 体积 质量 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.1387 mL 5.6933 mL 11.3865 mL 28.4663 mL
5 mM 0.2277 mL 1.1387 mL 2.2773 mL 5.6933 mL
10 mM 0.1139 mL 0.5693 mL 1.1387 mL 2.8466 mL

计算器

摩尔浓度计算器
稀释计算器
配液计算器
分子量计算器
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参考文献

1. Lee J H , Lee J S , Kim S , et al. Comparison of cytotoxicities and wound healing effects of diquafosol tetrasodium and hyaluronic acid on Human corneal epithelial cells[J]. Korean Journal of Physiology and Pharmacology, 2017, 21(2):189. 2. Fujihara T , Murakami T , Fujita H , et al. Improvement of Corneal Barrier Function by the P2Y2 Agonist INS365 in a Rat Dry Eye Model[J]. Investigative Ophthalmology & Visual Science, 2001, 42(1):96-100.

文献引用

1. Zhao Y, Li Y, Zhu R, et al.RPS15 interacted with IGF2BP1 to promote esophageal squamous cell carcinoma development via recognizing m6A modification.Signal Transduction and Targeted Therapy.2023, 8(1): 224.
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相关化合物库

该产品包含在如下化合物库中:
抗癌临床化合物库 抗癌上市药物库 抗癌药物库 药物功能重定位化合物库 经典已知活性库 抗COVID-19化合物库 GPCR靶点分子库 神经信号分子库 已知活性化合物库 核苷类化合物库

剂量换算

对于不同动物的给药剂量换算,您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。

体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。

第一步:请输入动物实验的基本信息
剂量
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g
给药体积
μL
动物数量
第二步:请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
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技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

Diquafosol tetrasodium 211427-08-6 GPCR/G Protein Neuroscience P2Y Receptor Inhibitor inhibit INS 365 地夸磷索四钠 Diquafosol INS-365 INS365 inhibitor

 

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