Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Diphenyleneiodonium chloride (DPI) 是 NADPH 氧化酶抑制剂,也是 TRPA1激活剂,EC50为 1 - 3 μM 。它选择性抑制胞内活性氧。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 179 | 现货 | ||
5 mg | ¥ 398 | 现货 | ||
10 mg | ¥ 638 | 现货 | ||
25 mg | ¥ 1,160 | 现货 | ||
50 mg | ¥ 1,890 | 现货 | ||
100 mg | ¥ 3,230 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 452 | 现货 |
产品描述 | Diphenyleneiodonium chloride (DPI)(DPI) is an irreversible inhibitor of iNOS and eNOS (IC50 values of 50 nM and 0.3 μM, respectively),and displays broad-spectrum bactericidal activity. |
靶点活性 | iNOS:50 nM , eNOS:0.3 µM |
体外活性 | Diphenyleneiodonium chloride (DPIC), an NADPH/NADH oxidase inhibitor, as possessing potent antimicrobial activity against non-tuberculous mycobacteria.?DPIC exhibited concentration-dependent bactericidal activity against M. fortuitum and synergized with amikacin, ceftriaxone, ceftazidime and meropenem[1]. |
细胞实验 | Whole-cell growth inhibition assays were used to screen and identify novel inhibitors. The hit compounds(Diphenyleneiodonium chloride) were tested for cytotoxicity against Vero cells to determine the selectivity index, and time-kill kinetics were determined against Mycobacterium fortuitum. The compound's ability to synergize with amikacin, ceftriaxone, ceftazidime and meropenem was determined using fractional inhibitory concentration indexes followed by its ability to decimate mycobacterial infections ex vivo[1]. |
别名 | 二苯基氯化碘盐, DPI |
分子量 | 314.55 |
分子式 | C12H8ClI |
CAS No. | 4673-26-1 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 6 mg/mL (19.07 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.1791 mL | 15.8957 mL | 31.7914 mL | 79.4786 mL |
5 mM | 0.6358 mL | 3.1791 mL | 6.3583 mL | 15.8957 mL | |
10 mM | 0.3179 mL | 1.5896 mL | 3.1791 mL | 7.9479 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Diphenyleneiodonium chloride 4673-26-1 Immunology/Inflammation Membrane transporter/Ion channel Metabolism NF-Κb NOS Reactive Oxygen Species NADPH TRP/TRPV Channel NADPH Oxidase Diphenyleneiodonium inhibit NOX Diphenyleneiodonium Chloride 二苯基氯化碘盐 TRP Channel DPI Transient receptor potential channels Inhibitor inhibitor