Didox

产品编号: T7525 别名:

3,4-二羟基苯甲羟肟酸,NSC-324360

Didox 是一种合成的核糖核苷酸还原酶抑制剂，可降低患有痴呆症的 HIV 患者大脑中氧化损伤标志物的水平。

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CAS：69839-83-4

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生物活性化学信息储存和溶解度信息参考文献引用文献

产品描述

Didox is a synthetic ribonucleotide reductase (RR) inhibitor that has been found to reduce the levels of oxidative injury markers in the brains of HIV patients with dementia.

体外活性

Didox induced cell death and that this effect was suppressed by iron supplementation.?Cell treatments with didox caused changes of cellular iron content, TfR1 and ferritin levels comparable to those caused by the iron chelators, deferoxamine (DFO) and deferiprone (DFP).Didox is a bidentated iron chelator with two theoretical possible positions for the binding and among them that with the two hydroxyls of the catechol group acting as ligands is the more likely one. The iron chelating property of didox may contribute to its antitumor activity not only blocking the formation of the tyrosil radical on Tyr122 (such as HU) on RRM2 (essential for its activity) but also sequestering the iron needed by this enzyme and to the cell proliferation[1].

体内活性

Didox treatment of mouse bone marrow-derived mast cells (BMMC) reduced IgE-stimulated degranulation and cytokine production, including IL-6, IL-13, TNF and MIP-1a (CCL3)[2].

细胞实验

The cells were seeded in a 96-well plate (at a density of 2 × 10^3 cells for HA22T/VGH;?1.5 × 103 cells for HuH7) and exposed to various concentrations of didox and only HA22T/VGH also to hydroxyurea, DFO or DFP (0, 1, 10, 25, 50, 100, 200 and 500 μM) for 24, 48 and 72 h. In other experiments, HA22T/VGH were seeded in 96-well plates and treated with a single dose of didox, HU, DFO, DFP alone or in combination with increasing doses of FAC (25, 50, 100, 200 and 400 μM) for 48–72 h. In other type of treatment,?HA22T/VGH cells were or pre-treated for 16 h with a single dose of didox (200 μM) and then treated in combination with FAC (400–800 μM) or directly in combination didox-FAC for 48–72 h.Cell viability was evaluated with an MTT assay.?After the indicated time points and treatments, the supernatant was removed and 100 μL of the MTT solution (0.5 mg/mL) diluted in the cell medium was added to the wells.?After 3.5 h of incubation at 37 °C and 5% CO2, the MTT medium was removed and 75 μL of DMSO was added to each well.?Plates were shaken for 15 min at 37 °C until complete dissolution and absorbance was measured at 540 nm emission wavelengths.?Average percentage of cell viability at each concentration was calculated using Microsoft Excel 2016 software[1].

Cas No.

69839-83-4

分子式

C7H7NO4

分子量

169.136

别名

3,4-二羟基苯甲羟肟酸;NSC-324360;Didox

参考文献

[1]Asperti M, Cantamessa L, Ghidinelli S,et al.The Antitumor Didox Acts as an Iron Chelator in Hepatocellular Carcinoma Cells[1].Pharmaceuticals (Basel). 2019 Sep 2;12(3).[2]Mcleod J J A , Caslin H L , Spence A J , et al. Didox (3,4-dihydroxybenzohydroxamic acid) suppresses IgE-mediated mast cell activation through attenuation of NFκB and AP-1 transcription[J]. Cellular Immunology, 2017:S0008874917301442.

储存和溶解度

DMSO:100 mg/mL (591.26 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years

剂量换算 Didox 69839-83-4

对于各种应用，安全且有效的用药剂量是很有必要的了解更多

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第一步：请输入动物实验的基本信息

剂量 mg/kg

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第二步：请输入动物体内配方组成，不同的产品配方组成不同，如有配方需求，可先联系我们提供正确的体内配方。

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% ddH₂O

% DMSO +

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计算结果如下:

工作液浓度: mg/ml;

母液配置方法: mg 药物溶于 μL DMSO (母液浓度为 mg/mL)，如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法：取 μL DMSO 主液，加入 μL PEG300，混匀澄清，再加 μL Tween 80，混匀澄清，再加 μL ddH₂O, 混匀澄清。

备注:

要按顺序从左向右依次添加助溶剂。可配合物理方法，如涡流、超声波或热水浴使之帮助溶解。

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技术支持

在抑制剂处理说明中可以找到您可能遇到的问题的答案。主题包括如何准备储备溶液，如何存储产品以及基于细胞的测定和动物实验需要特别注意的问题。

Keywords

Inhibitor DNA/RNA Synthesis 3,4-二羟基苯甲羟肟酸 inhibit Didox NSC-324360 NSC 324360 NSC324360 69839-83-4

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