Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Diclofenac diethylamine 是非选择性抗炎剂,通过活化 caspase 级联反应来诱导神经干细胞凋亡。它是一种COX 抑制剂,在 CHO 细胞中,对人COX-1和COX-2的IC50值分别为 4 和 1.3 nM。它对绵羊COX-1和COX-2的IC50值分别为 5.1 μM 和0.84 μM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
500 mg | ¥ 291 | 现货 | ||
5 g | ¥ 393 | 现货 | ||
10 g | ¥ 655 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 418 | 现货 |
产品描述 | Diclofenac Diethylamine, a non-selective COX inhibitor, is utilized as a nonsteroidal anti-inflammatory drug (NSAID). |
靶点活性 | hμMan COX-2(in CHO cells):1.3 nM, human COX-1(in CHO cells):4 nM, Ovine COX-1:5.1μM, Ovine COX-2:0.84 μM |
体外活性 | The primary mechanism of diclofenac diethylamine responsible for its anti-inflammatory, antipyretic, and analgesic action is thought to be inhibition of prostaglandin synthesis by inhibition of cyclooxygenase (COX). It also appears to exhibit bacteriostatic activity by inhibiting bacterial DNA synthesis. Inhibition of COX also decreases prostaglandins in the epithelium of the stomach, making it more sensitive to corrosion by gastric acid. This is also the main side effect of diclofenac. Diclofenac diethylamine has a low to moderate preference to block the COX2-isoenzyme (approximately 10-fold) and is said to have, therefore, a somewhat lower incidence of gastrointestinal complaints than noted with indomethacin and aspirin. |
激酶实验 | HDAC Activity: HDAC activity is measured using a continuous trypsin-coupled assay. For inhibitor characterization, measurements are done in a reaction volume of 100 μL using 96-well assay plates. For each isozyme, the HDAC protein in reaction buffer [50 mM HEPES, 100 mM KCl, 0.001% Tween 20, 5% DMSO (pH 7.4), supplemented with bovine serum albumin at concentrations of 0% (HDAC1), 0.01% (HDAC2, 3, 8, and 10), or 0.05% (HDAC6)] is mixed with PCI-24781 at various concentrations and allowed to incubate for 15 minutes. Trypsin is added to a final concentration of 50 nM, and acetyl-Gly-Ala-(N-acetyl-Lys)-AMC is added to a final concentration of 25 μM (HDAC1, 3, and 6), 50 μM (HDAC2 and 10), or 100 μM (HDAC8) to initiate the reaction. Negative control reactions are done in the absence of PCI-24781 in replicates of eight. Reactions are monitored in a fluorescence plate reader. After a 30-minute lag time, the fluorescence is measured over a 30-minute time frame using an excitation wavelength of 355 nm and a detection wavelength of 460 nm. The increase in fluorescence with time is used as the measure of the reaction rate. Inhibition constants Ki(app) are obtained using the program BatchKi. |
分子量 | 369.29 |
分子式 | C18H22Cl2N2O2 |
CAS No. | 78213-16-8 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: 69 mg/mL (186.8 mM)
H2O: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 69 mg/mL (186.8 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
Ethanol / DMSO | 1 mM | 2.7079 mL | 13.5395 mL | 27.079 mL | 67.6975 mL |
5 mM | 0.5416 mL | 2.7079 mL | 5.4158 mL | 13.5395 mL | |
10 mM | 0.2708 mL | 1.3539 mL | 2.7079 mL | 6.7697 mL | |
20 mM | 0.1354 mL | 0.677 mL | 1.3539 mL | 3.3849 mL | |
50 mM | 0.0542 mL | 0.2708 mL | 0.5416 mL | 1.3539 mL | |
100 mM | 0.0271 mL | 0.1354 mL | 0.2708 mL | 0.677 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Diclofenac diethylamine 78213-16-8 Apoptosis Immunology/Inflammation Neuroscience COX Cyclooxygenase anti-inflammatory cascade CHO COX-1 neural Inhibitor NSCs stem ovine caspase inhibit cells COX-2 Diclofenac inhibitor