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Daunorubicin hydrochloride

Daunorubicin hydrochloride

产品编号 T1511   CAS 23541-50-6
别名: Daunomycin HCl, 盐酸佐柔比星, 盐酸柔红霉素, RP-13057 Hydrochloride, Rubidomycin hydrochloride, Daunorubicin HCl, Daunomycin

Daunorubicin hydrochloride (Rubidomycin hydrochloride) 是一种蒽环类氨基糖苷类抗肿瘤药物,可抑制 DNA 复制和修复以及 RNA 和蛋白质合成。

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Daunorubicin hydrochloride Chemical Structure
Daunorubicin hydrochloride, CAS 23541-50-6
规格 价格/CNY 货期 数量
5 mg ¥ 262 现货
10 mg ¥ 426 现货
25 mg ¥ 769 现货
50 mg ¥ 1,330 现货
100 mg ¥ 1,920 现货
200 mg ¥ 2,890 现货
500 mg ¥ 4,880 现货
1 mL * 10 mM (in DMSO) ¥ 469 现货
其他形式的 Daunorubicin hydrochloride:
产品目录号及名称: Daunorubicin hydrochloride (T1511)
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纯度: 99.53%
纯度: 98.60%
纯度: 96.21%
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天然产物信息
生物活性
化学信息
存储 & 溶解度
参考文献
产品描述 Daunorubicin hydrochloride (Rubidomycin hydrochloride), an anthracycline aminoglycoside antineoplastic, inhibits DNA replication and repair and RNA and protein synthesis.
靶点活性 DNA synthesis:20 nM(Ki)
体外活性 At drug concentrations that reflect the peak plasma concentration after Daunorubicin administration, the primary mechanism is likely to be through interaction with topoisomerase II, which may be a primary triggering event for growth arrest and/or cell killing through a signalling pathway leading to apoptosis, at least in leukemic cells and thymocytes. The quinone structure permits daunorubicin to act as electron acceptors in reactions mediated by oxoreductive enzymes including cytochrome P450 reductase, NADH dehydrogenase, and xanthine oxidase. At Daunorubicin concentrations exceeding approximately 2–4 μM, free radical mediated toxicity and DNA cross-linking may become evident. Daunorubicin inhibits both DNA and RNA syntheses in HeLa cells over a concentration range of 0.2 through 2 μM. Daunorubicin inhibits both DNA syntheses in Ehrlich ascites tumor cells over a concentration range of 4 μM. Daunorubic triggers apoptosis at concentrations of 0.5 and 1 μM in either HL-60 or U-937 human leukemic cells. [1] Daunorubicin stimulates ceramide elevation and apoptosis in P388 and U937 cells through de novo synthesis via activation of the enzyme ceramide synthase. [2] Daunorubicin dose-dependently increases the phosphatidylserine exposure and consequent procoagulant activity of human umbilical vein endothelial cells. Daunorubicin (0.2 mM) significantly enhances the release of endothelial microparticles which are highly procoagulant in human umbilical vein endothelial cells. [3]
体内活性 Urinary protein excretion, serum creatinine, and blood urea nitrogen (BUN) level are significantly increased in group Daunorubicin (3 mg/kg, i.v.) compared with those in group Control. Administration of Daunorubicin (DNR) causes a significant increase in malondialdehyde (MDA) level in renal tissue compared with that in the control group[5].
细胞实验 Daunorubicin (Dnr) is prepared in PBS[2]. The chemosensitivity to Daunorubicin is assessed using the MTT assay. In brief, the 96 well plates are set up with cells at the initial density of 2×105 cells/mL and are incubated at 37°C for 72 h in an atmosphere of 5% CO2 in the absence and presence of nine different concentrations of Daunorubicin (Dnr) or Dox ranging from 1.90 to 0.007 μM in triplicate. After incubation, 10 μL of MTT solution (5 mg/mL tetrazolium salt) is added to each well and the plates are incubated for a further 4 h at 37°C. The formazan salt crystals are dissolved by adding 100 μL 10% SDS in 10 mM HCl solution and incubating over night at 37°C. The absorbance is measured at 540 nm with a reference at 650 nm by a 96-well enzyme-linked immunosorbent assay (ELISA) plate reader. Chemosensitivity is expressed as the IC50, which is the concentration of drug causing 50% cell survival compare to control cells grown without drug. Calculations are carried out using Microsoft Excel[2].
别名 Daunomycin HCl, 盐酸佐柔比星, 盐酸柔红霉素, RP-13057 Hydrochloride, Rubidomycin hydrochloride, Daunorubicin HCl, Daunomycin
分子量 563.99
分子式 C27H29NO10·HCl
CAS No. 23541-50-6

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

H2O: 92 mg/mL (163.1 mM)

DMSO: 93 mg/mL (164.9 mM)

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

溶液配制表

可选溶剂 浓度 体积 质量 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 1.7731 mL 8.8654 mL 17.7308 mL 44.327 mL
5 mM 0.3546 mL 1.7731 mL 3.5462 mL 8.8654 mL
10 mM 0.1773 mL 0.8865 mL 1.7731 mL 4.4327 mL
20 mM 0.0887 mL 0.4433 mL 0.8865 mL 2.2164 mL
50 mM 0.0355 mL 0.1773 mL 0.3546 mL 0.8865 mL
100 mM 0.0177 mL 0.0887 mL 0.1773 mL 0.4433 mL

计算器

摩尔浓度计算器
稀释计算器
配液计算器
分子量计算器
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输入分子式,点击计算,可计算出产品的分子量。

参考文献

1. Gewirtz DA, et al. Biochem Pharmacol, 1999, 57(7), 727-741. 2. Bose R, et al. Cell, 1995, 82(3), 405-414. 3. Fu Y, et al. Thromb Haemost, 2010, 104(6), 1235-1241. 4. Tardi P, et al. Leuk Res, 2009, 33(1), 129-139. 5. Arozal W, et al. Telmisartan prevents the progression of renal injury in daunorubicin rats with the alteration of angiotensin II and endothelin-1 receptor expression associated with its PPAR-γ agonist actions. Toxicology. 2011 Jan 11;279(1-3):91-9.

文献引用

1. Wang B, He B S, Ruan X L, et al. An integrated microfluidics platform with high-throughput single-cell cloning array and concentration gradient generator for efficient cancer drug effect screening. Military Medical Research. 2022, 9(1): 1-17. 2. Idris N, Leong K H, Wong E H, et al.Unveiling synergism of polymyxin B with chloramphenicol derivatives against multidrug-resistant (MDR) Klebsiella pneumoniae.The Journal of Antibiotics.2023: 1-9.
Pirarubicin 9-Chloromethyl-10-hydroxy-11-F-Camptothecin Lupalbigenin Oxaquin TEA Topoisomerase I inhibitor 10 Lucidin primeveroside Isosteviol Doxorubicin

相关化合物库

该产品包含在如下化合物库中:
抗癌临床化合物库 抗癌活性化合物库 抗癌药物库 抗癌上市药物库 微生物天然产物库 造血毒性小分子库 已知活性化合物库 DNA 损伤和修复分子库 经典已知活性库 抗癌天然产物库

剂量换算

对于不同动物的给药剂量换算,您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。

体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。

第一步:请输入动物实验的基本信息
剂量
mg/kg
每只动物体重
g
给药体积
μL
动物数量
第二步:请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
% DMSO
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% Tween 80
% ddH2O
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技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

Daunorubicin hydrochloride 23541-50-6 Apoptosis Autophagy Cell Cycle/Checkpoint DNA Damage/DNA Repair Microbiology/Virology DNA/RNA Synthesis Topoisomerase Antibacterial Antibiotic RP13057 pancreatic carcinoma leukemia K562 cells L3.6 cell Ehrlich ascites tumor chamomile RP 13057 Hydrochloride RP-13057 Inhibitor Daunomycin HCl 盐酸佐柔比星 盐酸柔红霉素 RP13057 Hydrochloride Rubidomycin ADC Payload Anti-tumor Rubidomycin Hydrochloride inhibit Molt-4 cell ADC Cytotoxin Daunorubicin cytotoxin RP 13057 RP-13057 Hydrochloride Bacterial Rubidomycin hydrochloride Daunorubicin Hydrochloride Daunorubicin HCl Daunomycin inhibitor

 

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