Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DB07268 是选择性JNK1抑制剂,IC50值为 9 nM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 461 | 现货 | ||
2 mg | ¥ 688 | 现货 | ||
5 mg | ¥ 1,230 | 现货 | ||
10 mg | ¥ 1,930 | 现货 | ||
25 mg | ¥ 3,810 | 现货 | ||
50 mg | ¥ 5,470 | 现货 | ||
100 mg | ¥ 7,670 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 878 | 现货 |
产品描述 | DB07268 is a potent and selective JNK1 inhibitor. |
靶点活性 | JNK1:9 nM. |
激酶实验 | Following 3T3L1 adipocyte serum-starvation and insulin stimulation, cell lysates containing protease inhibitors are clarified and then subjected to immunoprecipitation with anti-PIKfyve antibodies. Washed beads are mixed with 100 μM PtdIns and preincubated for 15 min with YM-201636 (100 nM) or vehicle in the assay buffer (50 mM Tris-HCl, pH 7.5, 1 mM EGTA and 10 mM MgCl2). The kinase assay (50 μL final volume) is carried out for 15 min at 37 °C with 15 μM ATP and [γ-32P]ATP (30 μCi). Lipids are extracted, spotted on TLC glass plates (250 μm), resolved by a chloroform/methanol/water/ammonia solvent system and detected by autoradiography[2]. |
分子量 | 321.33 |
分子式 | C17H15N5O2 |
CAS No. | 929007-72-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 50 mg/mL (155.6 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.1121 mL | 15.5603 mL | 31.1207 mL | 77.8016 mL |
5 mM | 0.6224 mL | 3.1121 mL | 6.2241 mL | 15.5603 mL | |
10 mM | 0.3112 mL | 1.556 mL | 3.1121 mL | 7.7802 mL | |
20 mM | 0.1556 mL | 0.778 mL | 1.556 mL | 3.8901 mL | |
50 mM | 0.0622 mL | 0.3112 mL | 0.6224 mL | 1.556 mL | |
100 mM | 0.0311 mL | 0.1556 mL | 0.3112 mL | 0.778 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
DB07268 929007-72-7 MAPK JNK DB 07268 DB-07268 Inhibitor inhibit inhibitor