Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Cyclobenzaprine hydrochloride (MK130 hydrochloride) 是一种具有抗抑郁活性的中枢作用肌肉松弛剂,可减少肌肉痉挛。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 mg | ¥ 100 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 183 | 现货 |
产品描述 | Cyclobenzaprine hydrochloride (MK130 hydrochloride) , a centrally acting muscle relaxant with antidepressant activity, leads to a reduction in muscle spasms. |
激酶实验 | DNA synthesis assay : The rate of DNA synthesis is measured by the incorporation of radioactive thymidine into DNA. HL-60 and KG1a cells are suspended in 2 mL RPMI medium containing 10% fetal serum in 6-well (35 mm diameter) dishes and incubated with different concentrations of corresponding drugs for 48 hours (drugs are added simultaneously). At 48 hours, 0.5 μCi [3H] thymidine (6.7 Ci/mmol) is added to each well and incubated for an additional 24 hours. The cells are placed on GF/C glass fiber filters (2.4 cm diameter), washed with cold 0.9% NaCl, 5% cold trichloroacetic acid and ethanol. The filters containing the DNA are then dried, placed in EcoLite scintillation liquid (ICN) and the radioactivity measured using Beckman LS 6000IC scintillation counter. The IC50 is defined as the concentration of drug that inhibits 50% of the DNA synthesis of the leukemic cell lines from the dose–response curve. |
别名 | 盐酸环苯扎林, Cyclobenzaprine HCl, MK130 hydrochloride |
分子量 | 311.86 |
分子式 | C20H22ClN |
CAS No. | 6202-23-9 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: 57 mg/mL (182.8 mM)
DMSO: 58 mg/mL (186 mM)
Ethanol: 58 mg/mL (186 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
H2O / DMSO / Ethanol | 1 mM | 3.2066 mL | 16.0328 mL | 32.0657 mL | 80.1642 mL |
5 mM | 0.6413 mL | 3.2066 mL | 6.4131 mL | 16.0328 mL | |
10 mM | 0.3207 mL | 1.6033 mL | 3.2066 mL | 8.0164 mL | |
20 mM | 0.1603 mL | 0.8016 mL | 1.6033 mL | 4.0082 mL | |
50 mM | 0.0641 mL | 0.3207 mL | 0.6413 mL | 1.6033 mL | |
100 mM | 0.0321 mL | 0.1603 mL | 0.3207 mL | 0.8016 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Cyclobenzaprine hydrochloride 6202-23-9 GPCR/G Protein Neuroscience 5-HT Receptor 盐酸环苯扎林 inhibit Cyclobenzaprine Hydrochloride MK130 MK 130 Hydrochloride MK-130 Hydrochloride Cyclobenzaprine HCl MK-130 Inhibitor 5-hydroxytryptamine Receptor MK130 hydrochloride Cyclobenzaprine Serotonin Receptor MK130 Hydrochloride MK 130 inhibitor