Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Cridanimod (10-carboxymethyl-9-acridanone, CMA) 是一种是小分子免疫调节剂和干扰素诱导剂,是一种孕酮受体激活剂,能够诱导 IFNα和IFNβ的表达,显著增加 PR 的表达。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 248 | 现货 | ||
5 mg | ¥ 568 | 现货 | ||
10 mg | ¥ 897 | 现货 | ||
25 mg | ¥ 1,480 | 现货 | ||
50 mg | ¥ 2,230 | 现货 | ||
100 mg | ¥ 3,330 | 现货 | ||
500 mg | ¥ 7,280 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 555 | 现货 |
产品描述 | Cridanimod (10-carboxymethyl-9-acridanone, CMA) is a potent STING agonist that directly binds to STING and triggers a strong antiviral response through the TBK1/IRF3 route. |
靶点活性 | mSTING:3.5 μM (Kd) |
体外活性 | CMA induced robust IRF3 phosphorylation that was followed by strong Ifnb mRNA induction and translation. CMA-mediated IFNβ production reached peak levels already 4?h after stimulation, even exceeding LPS in its readiness to trigger IFNβ synthesis [1]. rSTING and hSTING do not respond or only respond weakly to CMA, whereas it strongly activated mSTING. Endogenous mRNA level of Ifnb, Cxcl10, and Il6 were robustly increased when treated with CMA in murine macrophages, but not in rat macrophages and human THP-1 cells [2]. |
细胞实验 | For transfection experiments, primary macrophages were seeded with a density of 1 × 10^5?per?ml. Cells were transfected with poly(I:C) (2?μg/ml), pppRNA (1.33?μg/ml), ISD (2?μg/ml) and c-diGMP (8.66?μg/ml) using Lipofectamine 2000, according to the manufacturer's instructions. LPS (200?ng/ml) and CMA were directly added to the medium. Human PBMCs were transfected as described above, at a density of 4 × 10^6?per?ml, human fibroblasts at a density of 1.5 × 105?per?ml. For western blot experiments, cells were lysed after 2?h, if not indicated otherwise. For cytokine assays, supernatants were collected after 18–20?h. For RNA isolation, cells were lysed after 4?h [1]. |
别名 | CMA, 吖啶酮乙酸, XBIO-101, 10-carboxymethyl-9-acridanone |
分子量 | 253.25 |
分子式 | C15H11NO3 |
CAS No. | 38609-97-1 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 25 mg/mL (98.7 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.9487 mL | 19.7433 mL | 39.4867 mL | 98.7167 mL |
5 mM | 0.7897 mL | 3.9487 mL | 7.8973 mL | 19.7433 mL | |
10 mM | 0.3949 mL | 1.9743 mL | 3.9487 mL | 9.8717 mL | |
20 mM | 0.1974 mL | 0.9872 mL | 1.9743 mL | 4.9358 mL | |
50 mM | 0.079 mL | 0.3949 mL | 0.7897 mL | 1.9743 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Cridanimod 38609-97-1 Immunology/Inflammation Others Progesterone Receptor STING IFNAR XBIO101 inhibit Interferon-α/β receptor CMA XBIO 101 NR3C3 Interferon-alpha/beta receptor 吖啶酮乙酸 XBIO-101 Inhibitor 10-carboxymethyl-9-acridanone inhibitor