Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Cortisone acetate (NSC-49420) 是 Cortisol(一种糖皮质激素)的氧化代谢产物。它在高浓度下能够部分干预 Glucocorticoid 与Glucocorticoid-receptor 的结合。它具有免疫抑制和消炎的作用。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
500 mg | ¥ 148 | 现货 | ||
1 g | ¥ 198 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 210 | 现货 |
产品描述 | Cortisone Acetate is a synthetic or semisynthetic analog of the naturally occurring cortisone hormone produced by the adrenal glands with anti-inflammatory and immunomodulating properties. Cortisone acetate (NSC-49420) diffuses through the cell membrane and binds to nuclear glucocorticoid receptors. The receptor-ligand complex binds to promotor regions of certain genes and initiates RNA transcription. This results in an induction of synthesis of certain anti-inflammatory proteins while inhibiting the synthesis of certain inflammatory mediators. |
激酶实验 | Levels of enzymatically active caspase-3 are quantified using the ApoAlert Caspase Fluorescent assay kit. Briefly, 1×106 BC-1 PEL cells are treated with 50 μM Miltefosine, 50 μM Perifosine, or 20 nM NVP-BEZ235, as well as the respective vehicle controls. Cells are harvested and lysed 12 hours later. Equivalent micrograms of cell lysate for all samples are incubated with a fluorogenic caspase-3 substrate (DEVD-AFC). Cleavage of DEVD by caspase-3 releases AFC, the fluorescence of which is measured using a FLUOstar OPTIMA fluorometer, with excitation and emission filter wavelengths set to 400 and 505 nm, respectively[3]. |
别名 | 醋酸可的松, Cortisone 21-acetate, NSC 49420 |
分子量 | 402.48 |
分子式 | C23H30O6 |
CAS No. | 50-04-4 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 7 mg/mL (17.39 mM)
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.4846 mL | 12.423 mL | 24.846 mL | 62.1149 mL |
5 mM | 0.4969 mL | 2.4846 mL | 4.9692 mL | 12.423 mL | |
10 mM | 0.2485 mL | 1.2423 mL | 2.4846 mL | 6.2115 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Cortisone acetate 50-04-4 Endocrinology/Hormones Metabolism Endogenous Metabolite Glucocorticoid Receptor Cortisone immunosuppressant 醋酸可的松 Cortisone 21-acetate inhibit Cortisone Acetate anti-inflammatory NSC 49420 NSC49420 NSC-49420 Inhibitor inhibitor