Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Calcium dobesilate 具有血管保护特性,部分通过改善 HG 诱导的炎症来保护内皮细胞,用于慢性静脉疾病、糖尿病视网膜病变以及痔疮发作等研究。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
500 mg | ¥ 415 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 161 | 现货 |
产品描述 | Calcium dobesilate(CaD) has angioprotective properties and protects endothelial cells partly by ameliorating HG induced inflammation. |
体外活性 | CaD inhibited abnormal cell proliferation. high glucose (HG) treatment also significantly enhanced HVUEC migration compared to the control treatment. In contrast, CaD treatment partially inhibited HUVEC migration compared to HG exposure. HG?treated HUVECs exhibited increased FITC?BSA permeability compared to control cells cultured in medium alone; however, CaD application prevented the HG?induced increase in FITC?BSA permeability and suppressed HG?induced overexpression of endothelial markers (VEGF, VEGFR?2, endocan) and inflammation markers (ICAM?1, MCP?1, PTX3) in HUVECs. CaD has angioprotective properties and protects endothelial cells partly by ameliorating HG?induced inflammation[1]. |
细胞实验 | HUVECs were seeded in 96-well culture plates (1×10^4 cells/well). Following 24 h, the control or experimental medium was added. At 12, 24, 48 and 72 h, 10 μl CCK-8 reagent was added to each well for 1 h. Absorbance values were recorded at a wavelength of 450 nm using a microplate reader[1]. |
分子量 | 229.2 |
分子式 | C6H5O5SCa0.5 |
CAS No. | 20123-80-2 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 150 mg/mL (358.50 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 4.363 mL | 21.815 mL | 43.63 mL | 109.075 mL |
5 mM | 0.8726 mL | 4.363 mL | 8.726 mL | 21.815 mL | |
10 mM | 0.4363 mL | 2.1815 mL | 4.363 mL | 10.9075 mL | |
20 mM | 0.2182 mL | 1.0908 mL | 2.1815 mL | 5.4538 mL | |
50 mM | 0.0873 mL | 0.4363 mL | 0.8726 mL | 2.1815 mL | |
100 mM | 0.0436 mL | 0.2182 mL | 0.4363 mL | 1.0908 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Calcium dobesilate 20123-80-2 Apoptosis Others inhibit Inhibitor inhibitor