Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Calcipotriol monohydrate 是维生素 D3 的类似物,对维生素 D 受体具有高亲和力。Calcipotriol monohydrate 可用于银屑病研究。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 233 | 现货 | ||
5 mg | ¥ 523 | 现货 | ||
10 mg | ¥ 778 | 现货 | ||
25 mg | ¥ 1,550 | 现货 | ||
50 mg | ¥ 2,890 | 现货 | ||
100 mg | ¥ 4,370 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 619 | 现货 |
产品描述 | Calcipotriol monohydrate is an analog of Vitamin D3 with a high affinity for the vitamin D receptor. Calcipotriol monohydrate can be used for studies about psoriasis. |
体外活性 | Calcipotriol monohydrate(100, 10, 和 1 ng/mL)显著提升了NK细胞表面NKp30的表达量,在4小时的孵育后[1]。当NHEK细胞未被IL-17A或IL-22刺激时,Calcipotriol monohydrate轻微增强(0.2 nM)IL-8 mRNA表达,或无影响(2-20 nM)。加入IL-17A和IL-22后,IL-8的mRNA表达显著提升,证实了我们之前的研究。这种IL-8 mRNA表达的增强,通过Calcipotriol monohydrate(2, 20 和 40 nM)以剂量依赖方式被抑制[2]。 |
体内活性 | 在经过Diclofenac加Calcipotriol monohydrate(p=0.018)和Diclofenac加DFMO加Calcipotriol monohydrate(p=0.002)处理的组别中,与安慰剂(线性回归模型)[3]相比,体重增加显著较小。 |
细胞实验 | Normal human epidermal keratinocytes are grown in serum-free keratinocyte growth medium Epilife and used at the third passage in all experiments. The growth supplement is omitted 48 h before experiments. As a control, IL-17A and IL-22 are either added or not added to the cells. Cultured NHEK cells are stimulated with IL-17A (200 ng/mL) and/or IL-22 (200 ng/mL) followed by co-incubation in the presence or absence of Calcipotriol at 0.2-40 nM to test its modulatory effect. Cells are harvested 3 days later and subjected to real-time qPCR. Culture supernatants are also collected and frozen at -80°C until used for ELISA [1]. |
动物实验 | The 160 female SKH-1 hairless mice (6-7 weeks of age) are used. After UV treatment, mice without tumors are randomly divided into five groups, four chemoprevention groups (Diclofenac plus DFMO; Diclofenac plus Calcipotriol; DFMO plus calcitriol; and Diclofenac plus DFMO plus Calcipotriol) and one placebo group (skin lotion). The mice are treated with test mixtures once a day, five days a week, for a total of 17 weeks. The test mixtures are applied topically on the dorsal surface of the mice. Ten microliters are applied by a pipette after which the mixture is rubbed onto the skin. This corresponded to the following doses of each active substance in the treatments: 100 μg/week for Diclofenac (30 mg/g undiluted), 0.166 μg/week for Calcitriol (50 μg/g undiluted), and 463.3 μg/week for DFMO (139 mg/g undiluted) [3]. |
别名 | 钙泊三醇一水合物, 卡泊三醇一水合物 |
分子量 | 430.62 |
分子式 | C27H42O4 |
CAS No. | 147657-22-5 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: 95 mg/mL (220.61 mM), Sonication is recommended.
DMSO: 95 mg/mL (220.61 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
Ethanol / DMSO | 1 mM | 2.3222 mL | 11.6112 mL | 23.2223 mL | 58.0558 mL |
5 mM | 0.4644 mL | 2.3222 mL | 4.6445 mL | 11.6112 mL | |
10 mM | 0.2322 mL | 1.1611 mL | 2.3222 mL | 5.8056 mL | |
20 mM | 0.1161 mL | 0.5806 mL | 1.1611 mL | 2.9028 mL | |
50 mM | 0.0464 mL | 0.2322 mL | 0.4644 mL | 1.1611 mL | |
100 mM | 0.0232 mL | 0.1161 mL | 0.2322 mL | 0.5806 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Calcipotriol monohydrate 147657-22-5 VD/VDR Vitamin D receptor Calcipotriol Vitamin D Calcipotriol Monohydrate inhibit 钙泊三醇一水合物 Inhibitor 卡泊三醇一水合物 inhibitor