Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Cabotegravir (S/GSK1265744) 是一种HIV 整合酶抑制剂,可研究艾滋病。它抑制OAT1 和OAT3,IC50值为 0.81 和0.41 μM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
10 mg | ¥ 415 | 现货 | ||
25 mg | ¥ 878 | 现货 | ||
50 mg | ¥ 1,568 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 333 | 现货 |
产品描述 | Cabotegravir (S/GSK1265744) (GSK744, GSK1265744) is a long-acting HIV integrase inhibitor and inhibits the HIV-1 integrase catalyzed strand transfer reaction (IC50: 3.0 nM). |
靶点活性 | HIV-1:3.0 nM |
体内活性 | GSK1265744(50 mg/kg)可防止高剂量SHIV感染恒河猴. |
激酶实验 | In vitro strand transfer assay: The inhibitory concentrations of GSK1265744 is measured in a strand transfer assay using recombinant HIV IN. A complex of integrase and biotinylated donor DNA–streptavidin-coated SPA beads is formed by incubating 2 μM purified recombinant integrase with 0.66 μM biotinylated donor DNA–4 mg/mL streptavidin-coated SPA beads in 25 mM sodium MOPS pH 7.2, 23 mM NaCl, and 10 mM MgCl2 for 5 minutes at 37°C. These beads are spun down and preincubated with diluted INSTIs for 60 minutes at 37°C. Next, [3H]-labeled target DNA substrate is added to give a final concentration of 7 nM substrate, and the strand transfer reaction is incubated at 37°C for 25 to 45 minutes, which allowed for a linear increase in strand transfer of donor DNA to radiolabeled target DNA. The signal is read using a Wallac MicroBeta scintillation plate reader. |
别名 | GSK744, GSK-1265744, 卡博特韦, S/GSK1265744 |
分子量 | 405.35 |
分子式 | C19H17F2N3O5 |
CAS No. | 1051375-10-0 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 36 mg/mL (88.8 mM)
H2O: < 1 mg/mL (insoluble or slightly soluble)
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.467 mL | 12.335 mL | 24.67 mL | 61.6751 mL |
5 mM | 0.4934 mL | 2.467 mL | 4.934 mL | 12.335 mL | |
10 mM | 0.2467 mL | 1.2335 mL | 2.467 mL | 6.1675 mL | |
20 mM | 0.1234 mL | 0.6168 mL | 1.2335 mL | 3.0838 mL | |
50 mM | 0.0493 mL | 0.2467 mL | 0.4934 mL | 1.2335 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Cabotegravir 1051375-10-0 Microbiology/Virology Proteases/Proteasome HIV Protease Human immunodeficiency virus inhibit GSK744 GSK-1265744 HIV Integrase GSK-744 UGT1A1 OAT GSK 1265744 HIV 卡博特韦 S/GSK-1265744 Inhibitor S/GSK1265744 AIDS antiretroviral GSK1265744 GSK 744 S/GSK 1265744 inhibitor