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CW-069

CW-069

产品编号 T6209   CAS 1594094-64-0
别名: CW069

CW-069 是一种微管运动蛋白 HSET 的变构选择性抑制剂,IC50值为 75 μM,对 KSP 具有显着的特异性。

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CW-069 Chemical Structure
CW-069, CAS 1594094-64-0
规格 价格/CNY 货期 数量
1 mg ¥ 395 现货
2 mg ¥ 581 现货
5 mg ¥ 995 现货
10 mg ¥ 1,660 现货
25 mg ¥ 2,490 现货
50 mg ¥ 3,730 现货
100 mg ¥ 5,360 现货
500 mg ¥ 11,200 现货
1 mL * 10 mM (in DMSO) ¥ 995 现货
产品目录号及名称: CW-069 (T6209)
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选择批次  
纯度: 98.59%
纯度: 98.57%
纯度: 97.52%
纯度: 97.52%
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生物活性
化学信息
存储 & 溶解度
参考文献
产品描述 CW-069 (IC50=75 μM), an allosteric selective inhibitor of microtubule motor protein HSET, exhibits remarkable specificity over KSP.
靶点活性 HSET:75 μM
体外活性 CW069 increases multipolar spindles in N1E-115 cells with supernumerary centrosomes without altering bipolar spindle morphology in normal human dermal fibroblast cells. CW069 inhibits growth in cancer N1E-115 cells with IC50 of 10 μM, but not in NHDF or primary human bone marrow cells. [1]
激酶实验 In Vitro Enzymatic ATPase Assay: The protocol is optimized for use with full-length, N-terminal, 6His-tagged HSET and KSP, and measured the MT-stimulated activity of the proteins. Inhibition of the Gsp synthetase activity of HSET/KSP is observed spectrophotometrically by coupling the hydrolysis of ATP to oxidation of NADH via pyruvate kinase/lactate dehydrogenase reactions. The assay is initiated by adding purified Gsp synthetase/amidase (12.8 nM) to an assay mixture containing the following components (final concentration): 6 nM protein, 0.07 mg/ml MTs (University Biologicals), 1.56 mM glutathione, 10 mM spermidine, 2 mM ATP, 2.7 mM MgCl2, 1 mM phospho(enol)-pyruvate, 0.2 mM NADH, 50 μg/ml lactate dehydrogenase, 100 μg/ml pyruvate kinase, and various concentrations of inhibitor all in 50 mM Na PIPES (pH 6.8) at 37°C. The ADP-Glo detection assay (Promega) is performed as described in the manufacturer's instructions. All compound additions were performed using a multidrop BioMek Nxp. Plates were read using a Pherastar microplate reader.
细胞实验 Cells are cultured in DMEM supplemented with 10% fetal calf serum (FCS) at 37°C and 5% CO2. All compounds used in the Sulforhodamine B colorimetric (SRB) assay are dissolved in DMSO and diluted in culture medium to a final concentration of 0.2% DMSO. For the SRB assay and live-cell imaging, cells are seeded in 96-well plates at a density of 2,500 cells per well. After 24 hr, the cells are treated with compound for 72 hr, with triplicate wells for each concentration. For the SRB assay, the cells are then fixed with trichloroacetic acid (TCA) and stained with SRB. Fluorescence is quantified using an Infinite 200 PRO plate-reader at a wavelength of 545 nm. Compound-treated wells are compared with solvent control wells and the concentration of compound that results in 50% of the solvent-control cell growth is designated as the IC50 concentration, calculated using Graphpad PRISM 6. At least three biological replicates are performed for each assay.(Only for Reference)
别名 CW069
分子量 500.33
分子式 C23H21IN2O3
CAS No. 1594094-64-0

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

Ethanol: 4 mg/mL (7.99 mM)

DMSO: 93 mg/mL (185.9 mM)

H2O: < 1 mg/mL (insoluble or slightly soluble)

溶液配制表

可选溶剂 浓度 体积 质量 1 mg 5 mg 10 mg 25 mg
Ethanol / DMSO 1 mM 1.9987 mL 9.9934 mL 19.9868 mL 49.967 mL
5 mM 0.3997 mL 1.9987 mL 3.9974 mL 9.9934 mL
DMSO 10 mM 0.1999 mL 0.9993 mL 1.9987 mL 4.9967 mL
20 mM 0.0999 mL 0.4997 mL 0.9993 mL 2.4984 mL
50 mM 0.04 mL 0.1999 mL 0.3997 mL 0.9993 mL
100 mM 0.02 mL 0.0999 mL 0.1999 mL 0.4997 mL

计算器

摩尔浓度计算器
稀释计算器
配液计算器
分子量计算器
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输入分子式,点击计算,可计算出产品的分子量。

参考文献

1. Watts CA, et al. Chem Biol. 2013, 20(11), 1399-1410.
MPI-0479605 Paprotrain Eg5 Inhibitor V, trans-24 BRD9876 UMK57 BAY1217389 PVZB1194 (S)-Monastrol

相关化合物库

该产品包含在如下化合物库中:
微管靶向化合物库 抗癌活性化合物库 NO PAINS 化合物库 抑制剂库 抗衰老化合物库 经典已知活性库 抗癌化合物库 内质网应激化合物库 细胞骨架化合物库 已知活性化合物库

剂量换算

对于不同动物的给药剂量换算,您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。

体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。

第一步:请输入动物实验的基本信息
剂量
mg/kg
每只动物体重
g
给药体积
μL
动物数量
第二步:请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
% DMSO
%
% Tween 80
% ddH2O
计算 重置

技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

CW-069 1594094-64-0 Cytoskeletal Signaling Kinesin Microtubule Associated CW 069 Inhibitor inhibit CW069 inhibitor

 

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