Powder: -20°C for 3 years | In solvent: -80°C for 1 year
CPI-637 是一种选择性和细胞活性的苯二氮卓 CBP/EP300 溴结构域抑制剂,其对 CBP、EP300和 BRD4 BD-1的 IC50值分别为 0.03、0.051 和 11.0 μM,对 CBP 的 EC50值为 0.3 μM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 283 | 现货 | ||
2 mg | ¥ 395 | 现货 | ||
5 mg | ¥ 647 | 现货 | ||
10 mg | ¥ 913 | 现货 | ||
25 mg | ¥ 1,770 | 现货 | ||
50 mg | ¥ 3,230 | 现货 | ||
100 mg | ¥ 4,780 | 现货 | ||
500 mg | ¥ 9,870 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 647 | 现货 |
产品描述 | CPI-637 is a selective and cell-active benzodiazepinone CBP/EP300 bromodomain inhibitor. |
靶点活性 | CBP:0.03 μM, EP300:0.051 μM |
体外活性 | CPI-637 inhibits MYC expression in AMO-1 cells (EC50=0.60 μM)[1]. |
激酶实验 | Ki Determination: Inhibition of human full-length recombinant PARP-1 by [32P]NAD+ incorporation is measured. The [32P]ADP-ribose incorporated into acid insoluble material is quantified using a PhosphorImager. Ki is calculated by nonlinear regression analysis. |
细胞实验 | AMO-1 cells (DSMZ) were plated at 20,000 cells/well in 96-well plates in Iscove's Modified Dulbecco's Medium supplemented with 10% fetal calf serum. Cells were treated for 6 h with a dose titration of CPI-637 starting from a 5 μM top concentration. After 6 h, cells were lysed and processed for QuantiGene Plex expression analysis and data were collected on a MAGPIX multiplex Luminex instrument.(Only for Reference) |
别名 | CPI 637 |
分子量 | 386.45 |
分子式 | C22H22N6O |
CAS No. | 1884712-47-3 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 26 mg/mL (67.3 mM)
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
H2O: < 1 mg/mL (insoluble or slightly soluble)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.5877 mL | 12.9383 mL | 25.8766 mL | 64.6914 mL |
5 mM | 0.5175 mL | 2.5877 mL | 5.1753 mL | 12.9383 mL | |
10 mM | 0.2588 mL | 1.2938 mL | 2.5877 mL | 6.4691 mL | |
20 mM | 0.1294 mL | 0.6469 mL | 1.2938 mL | 3.2346 mL | |
50 mM | 0.0518 mL | 0.2588 mL | 0.5175 mL | 1.2938 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
CPI-637 1884712-47-3 Chromatin/Epigenetic Epigenetic Reader Domain Histone Acetyltransferase Inhibitor inhibit HAT HATs CPI637 CPI 637 inhibitor