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CFSE

产品编号 T6802 CAS 150347-59-4

别名: 5(6)-CFDA N-succinmidyl ester, Carboxyfluorescein succinimidyl ester, CFDA-SE, 5(6)-羧基二乙酸荧光素琥珀酰亚胺酯, 5(6)-Carboxyfluorescein diacetate succinimidyl ester

CFSE (CFDA-SE) 属于荧光染料，具有细胞膜渗透性。CFSE 可在活细胞内与胞内蛋白进行不可逆的结合，用于检测细胞增殖，标记的细胞呈绿色荧光，激发波长为 488 nm，发射波长为 518 nm。

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CFSE, CAS 150347-59-4

规格	价格/CNY	货期
1 mg	￥ 196	现货
5 mg	￥ 453	现货
10 mg	￥ 659	现货
25 mg	￥ 1,290	现货
50 mg	￥ 1,970	现货
100 mg	￥ 2,880	现货
200 mg	￥ 4,270	现货
500 mg	￥ 6,820	现货
1 mL * 10 mM (in DMSO)	￥ 783	现货

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选择批次

纯度: 99.13%

COA DATASHEET SDS HPLC HNMR

纯度: 98.98%

COA DATASHEET SDS HNMR HPLC

纯度: 97.94%

COA DATASHEET SDS HPLC HNMR

纯度: 95.91

COA DATASHEET SDS HNMR HPLC

DATASHEET SDS

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生物活性

化学信息

存储 & 溶解度

参考文献

产品描述	CFSE (CFDA-SE) is a fluorescent dye with cell membrane permeability. CFSE irreversibly binds to intracellular proteins in living cells and is used for the detection of cell proliferation. The labeled cells fluoresce in green color with excitation wavelength of 488 nm and emission wavelength of 518 nm.
体外活性	方法：将 1 mL 细胞和 CFDA (5 mM in 110μL PBS) 翻转混匀以标记细胞。CFSE 标记的 CD8+OT-I T 细胞与用不同量的 OVA 脉冲的树突细胞培养 3 天，使用 Flow Cytometry 检测 CFSE 图谱。结果：CD8+ T 细胞根据 CFSE 稀释峰分裂 1-3 次，更多的 T 细胞在较高的抗原浓度下分裂。[1] 方法：人红白血病细胞系K562、小鼠淋巴瘤细胞系YAC-1、人乳腺癌症细胞 MCF-7 和人黑色素瘤细胞系 A375 用 CFDA (1-10 mM) 处理 1-6 h，使用 Flow Cytometry 检测细胞死亡情况。结果：CFDA 对细胞无毒，因为 CFDA 标记引起的细胞死亡率低于 5%。[2]
体内活性	方法：CFSE 标记的 CD8+OT-I T 细胞尾静脉注射给 C56BL/6J 小鼠，随后静脉注射 OVA (20 μg)，三天后检测 CFSE 图谱。结果：大多数细胞落在 7 个 CFSE 峰值内，这表明细胞已经经历了多达 6 次分裂。[1] 方法：为在体内标记胸腺细胞，将 CFDA (10 μM) 注射到麻醉 C56BL/6 小鼠的胸腺叶。结果：CFDA 标记了所有胸腺细胞亚群的代表性样本，并且这些细胞以大约 2-3×10^6 个细胞/天的速率迁移到外周淋巴器官。它们在注射后第 1 天进入淋巴结，并停留至少 21 天，而在脾脏中的周转更快。[3]
细胞实验	The 5 mM CFDA-SE stock in DMSO is diluted to different concentrations(2 μM, 3 μM, 4 μM, 5 μM, 10 μM and 20 μM) in PBS with a total volume of 1 ml. After each cell line is harvested and washed three times with PBS, 1×109 cells are added to equal volume of CFDA-SE with different concentrations and incubated at 37°C for 5, 6, 7, 8, 10 and 15 min with agitation. The labeling reaction is stopped for 1 min by adding an equal volume of heat inactivated fetal bovine serum. The CFDA-SE labeled cells are washed twice with PBS and recounted, and the cell concentration is adjusted to 6×104cells/ml in IMDM containing 10% FCS.(Only for Reference)

别名	5(6)-CFDA N-succinmidyl ester, Carboxyfluorescein succinimidyl ester, CFDA-SE, 5(6)-羧基二乙酸荧光素琥珀酰亚胺酯, 5(6)-Carboxyfluorescein diacetate succinimidyl ester
分子量	557.46
分子式	C29H19NO11
CAS No.	150347-59-4

存储

keep away from direct sunlight　|　Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 93 mg/mL (166.8 mM)

H2O: < 1 mg/mL (insoluble or slightly soluble)

溶液配制表

可选溶剂	浓度体积质量	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.7939 mL	8.9693 mL	17.9385 mL	44.8463 mL
	5 mM	0.3588 mL	1.7939 mL	3.5877 mL	8.9693 mL
	10 mM	0.1794 mL	0.8969 mL	1.7939 mL	4.4846 mL
	20 mM	0.0897 mL	0.4485 mL	0.8969 mL	2.2423 mL
	50 mM	0.0359 mL	0.1794 mL	0.3588 mL	0.8969 mL
	100 mM	0.0179 mL	0.0897 mL	0.1794 mL	0.4485 mL

计算器

摩尔浓度计算器

稀释计算器

配液计算器

分子量计算器

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参考文献

1. Quah BJ, et al. The use of carboxyfluorescein diacetate succinimidyl ester (CFSE) to monitor lymphocyte proliferation. J Vis Exp. 2010 Oct 12;(44):2259. 2. Wang XQ, et al. Carboxyfluorescein diacetate succinimidyl ester fluorescent dye for cell labeling. Acta Biochim Biophys Sin (Shanghai). 2005 Jun;37(6):379-85. 3. Graziano M, et al. The fate of thymocytes labeled in vivo with CFSE. Exp Cell Res. 1998 Apr 10;240(1):75-85. 4. Li M, Qin M, Song G, et al. A biomimetic antitumor nanovaccine based on biocompatible calcium pyrophosphate and tumor cell membrane antigens[J]. Asian Journal of Pharmaceutical Sciences. 2020

文献引用

1. Guo E, Xiao R, Wu Y, et al. WEE1 inhibition induces anti-tumor immunity by activating ERV and the dsRNA pathway. Journal of Experimental Medicine. 2021, 219(1): e20210789. 2. Liao Y, Huang J, Liu P, et al. Downregulation of LNMAS orchestrates partial EMT and immune escape from macrophage phagocytosis to promote lymph node metastasis of cervical cancer. Oncogene. 2022: 1-13. 3. Xiao C, Su J, Zhang C, et al. Effectiveness of Booster Doses of the SARS-CoV-2 Inactivated Vaccine KCONVAC against the Mutant Strains. Viruses. 2022, 14(9): 2016. 4. Xiao C, Mao L, Wang Z, et al. SARS-CoV-2 variant B. 1.1. 7 caused HLA-A2+ CD8+ T cell epitope mutations for impaired cellular immune response. Iscience. 2022 Mar 18;25(3):103934. doi: 10.1016/j.isci.2022.103934. Epub 2022 Feb 17. 5. Deng J, Pan J, Qiu M, et al. Identification of HLA-A2 restricted CD8+ T cell epitopes in SARS-CoV-2 structural proteins. Journal of Leukocyte Biology. 2021 6. Jablonski S, Mou H, Otsuka Y, et al. Identification of Potent Small Molecule Inhibitors of SARS-CoV-2 Entry. SLAS Discovery. 2021 7. Zhao G, Tong Y, Xu J, et al.Jing-Fang powder ethyl acetate extracts attenuate atopic dermatitis by modulating T-cell activity.Molecular Immunology.2023, 160: 133-149. 8. Xiao C, Ren Z, Zhang B, et al.Insufficient epitope-specific T cell clones are responsible for impaired cellular immunity to inactivated SARS-CoV-2 vaccine in older adults.Nature Aging.2023: 1-18. 9. Yang L, Wu J, Mei G, et al.Corydalis Saxicola Bunting Total Alkaloid Eliminates Porphyromonas gingivalis strain 33277 Internalized into Macrophages by Inhibition of TLR2.Microbes and Infection.2023: 105244. 10. Li P, Xie Y, Wang J, et al.Gene engineered exosome reverses T cell exhaustion in cancer immunotherapy.Bioactive Materials.2024, 34: 466-481.

11. Qiu C, Peng B, Xiao C, et al.A novel method for identifying SARS-CoV-2 infection mutants via an epitope-specific CD8+ T cell test.Biosafety and Health.2024

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剂量换算

对于不同动物的给药剂量换算，您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL，一共给药动物10 只，您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法：2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL)，如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法：取 50 μL DMSO 主液，加入 300 μL PEG300，混匀澄清，再加 50 μL Tween 80，混匀澄清，再加 600 μL ddH2O, 混匀澄清。

第一步：请输入动物实验的基本信息

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给药体积

μL

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第二步：请输入动物体内配方组成，不同的产品配方组成不同，如有配方需求，可先联系我们提供正确的体内配方。

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