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CDDO-Im

CDDO-Im

产品编号 TQ0120   CAS 443104-02-7
别名: RTA-403, CDDO-Imidazolide, TP-235

CDDO-Im (TP-235) 是一种 Nrf2PPAR 的激活剂,对 PPARα 和 PPARγ 的 Ki 分别为232 nM 和 344 nM。

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CDDO-Im Chemical Structure
CDDO-Im, CAS 443104-02-7
规格 价格/CNY 货期 数量
1 mg ¥ 297 现货
5 mg ¥ 597 现货
10 mg ¥ 928 现货
25 mg ¥ 1,620 现货
50 mg ¥ 2,620 现货
100 mg ¥ 3,970 现货
500 mg ¥ 8,630 现货
1 mL * 10 mM (in DMSO) ¥ 712 现货
产品目录号及名称: CDDO-Im (TQ0120)
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纯度: 98.3%
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生物活性
化学信息
存储 & 溶解度
参考文献
产品描述 CDDO-Im (TP-235) is an activator of Nrf2 and PPAR (Kis: 232/344 nM for PPARα/PPARγ).
靶点活性 PPARγ:344 nM (Ki), PPARα:232 nM (Ki)
体外活性 CDDO-Im is highly active in suppressing cellular proliferation of human leukemia and breast cancer cell lines (IC50 approximately 10-30 nM). In U937 leukemia cells, CDDO-Im also induces monocytic differentiation as measured by increased cell surface expression of CD11b and CD36 [1]. CDDO-Im treatment markedly induces cell cycle arrest at G2/M-phase and apoptosis in the triple-negative breast cancer cell lines, SUM159 and MDA-MB-231. The CD24?/EpCAM+ cells in SUM159 tumorspheres are significantly inhibited by CDDO-Im treatment. CDDO-Im also significantly decreases sphere-forming efficiency and tumorsphere size in both primary and secondary sphere cultures [2]. Treatment with CDDO-Im elevates protein levels of Nrf2, a transcription factor previously shown to bind ARE sequences and increases expression of a number of antioxidant and detoxification genes regulated by Nrf2 [3].
体内活性 CDDO-Im has potent inhibitory against de novo inducible nitric oxide synthase expression in primary mouse macrophages. Moreover, CDDO-Im inhibits growth of B16 murine melanoma and L1210 murine leukemia cells in vivo. Injection of 10 nM (5.4 μg) of CDDO-Im almost completely blocks the ability of IFN-γ to induce iNOS, and treatment with as little as 1 nmol of CDDO-Im (0.54 μg) is partially effective [1].
细胞实验 CDDO-Im is dissolved in DMSO. SUM159 and MDA-MB-231 cells are seeded into each well of 96-well plates (1,000 cell/well) and treated the next day with vehicle control or CDDO-Im (1, 10, 50, 100 and 200 nM) for given incubation time. The absorbance is measured with a spectrophotometer to determine the cell proliferation rate [2].
动物实验 Mice are injected i.p. with thioglycollate, and the resulting resident peritoneal macrophages are activated 3 days later with an i.p. injection of IFN-γ. CDDO and CDDO-Im are injected i.p. 30 min after IFN-γ. Macrophages are harvested 10 h later, cultured for 12 h, and then assayed for expression of iNOS protein and production of nitric oxide (NO) [1].
别名 RTA-403, CDDO-Imidazolide, TP-235
分子量 541.72
分子式 C34H43N3O3
CAS No. 443104-02-7

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

DMSO: 50 mg/mL (92.30 mM), sonification is recommended.

H2O: Insoluble

溶液配制表

可选溶剂 浓度 体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.846 mL 9.2299 mL 18.4597 mL 46.1493 mL
5 mM 0.3692 mL 1.846 mL 3.6919 mL 9.2299 mL
10 mM 0.1846 mL 0.923 mL 1.846 mL 4.6149 mL
20 mM 0.0923 mL 0.4615 mL 0.923 mL 2.3075 mL
50 mM 0.0369 mL 0.1846 mL 0.3692 mL 0.923 mL

计算器

摩尔浓度计算器
稀释计算器
配液计算器
分子量计算器
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输入分子式,点击计算,可计算出产品的分子量。

参考文献

1. Place AE, et al. The novel synthetic triterpenoid, CDDO-imidazolide, inhibits inflammatory response and tumor growth in vivo. Clin Cancer Res. 2003 Jul;9(7):2798-806. 2. So JY, et al. A synthetic triterpenoid CDDO-Im inhibits tumorsphere formation by regulating stem cell signaling pathways in triple-negative breast cancer. PLoS One. 2014 Sep 17;9(9):e107616. 3. Liby K, et al. The synthetic triterpenoids, CDDO and CDDO-imidazolide, are potent inducers of heme oxygenase-1 and Nrf2/ARE signaling. Cancer Res. 2005 Jun 1;65(11):4789-98.
Antroquinonol Oltipraz (E)-[6]-Dehydroparadol Bardoxolone Methyl KI696 Hydrangenol Ginsenoside Rh3 Tigloylgomisin H

相关化合物库

该产品包含在如下化合物库中:
线粒体靶向库 细胞凋亡化合物库 铁死亡化合物库 NF-κB 通路分子库 DNA 损伤和修复分子库 抗胰腺癌化合物库 抗癌活性化合物库 抗糖尿病库 临床前化合物库 经典已知活性库

剂量换算

对于不同动物的给药剂量换算,您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。

体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。

第一步:请输入动物实验的基本信息
剂量
mg/kg
每只动物体重
g
给药体积
μL
动物数量
第二步:请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
% DMSO
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% ddH2O
计算 重置

技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

CDDO-Im 443104-02-7 Apoptosis DNA Damage/DNA Repair Immunology/Inflammation Metabolism Others Ferroptosis Nrf2 PPAR inhibit Keap1-Nrf2 RTA 403 CDDO Im Inhibitor TP 235 Peroxisome proliferator-activated receptors RTA-403 RTA403 CDDOIm TP235 CDDO-Imidazolide TP-235 inhibitor

 

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