Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Buparvaquone (BW 720C) 是与parvaquone 和atovaquone 有关的一种羟基萘醌类抗原虫药。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
5 mg | ¥ 207 | 现货 | ||
10 mg | ¥ 372 | 现货 | ||
50 mg | ¥ 544 | 现货 | ||
100 mg | ¥ 663 | 现货 | ||
500 mg | ¥ 1,652 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 414 | 现货 |
产品描述 | Buparvaquone (BW 720C) is a hydroxynaphthoquinone antiprotozoal drug related to atovaquone and parvaquone. |
体外活性 | Buparvaquone is significantly selective against L. (L.) infantum chagasi intracellular amastigotes, with an IC50 value of 1.5 μM. In 4-day proliferation assays, buparvaquone efficiently inhibits N.?caninum tachyzoite replication(IC50=4.9?nM; IC100=100?nM). Other cutaneous species are also susceptible to buparvaquone, with IC50 values in the range 1-4 μM. |
体内活性 | Both a hydrous gel and water-in-oil emulsion of buparvaquone significantly reduce cutaneous parasite burden and lesion size than the untreated control. Treatment of N. caninum infected mice with buparvaquone (100 mg/kg) either by intraperitoneal injection or gavage prevents neosporosis symptoms in 4 out of 6 mice in the intraperitoneally treated group, and in 6 out of 7 mice in the group receiving oral treatment. |
细胞实验 | To study whether pretreatment of host cells prior to invasion had any effect on parasite proliferation, confluent HFF grown in 6-well plates are treated with 1?μM buparvaquone in medium for 1?h or 5?h, and controls are exposed to the corresponding amounts of DMSO. Subsequently, the drug-containing medium is removed and monolayers are ished 4 times with Hank's Balanced Salt Solution, and are infected with Nc-Liv tachyzoites in 5?mL medium without any drug or solvent. After 2 days, cells are collected with a cell scraper, centrifuged, ished once more in PBS, and the pellet is stored at ?20?°C prior to quantification of N.?caninum proliferation by N.?caninum-specific real time PCR as outlined below. |
动物实验 | Mice: On day 0, all mice are infected by intraperitoneal (i.p.) injection of freshly purified N.?caninum tachyzoites. After 48?h, mice receive BPQ (100?mg/kg) as suspension in corn oil either by i.p. injection of a volume of 100?μl or by oral application of 100?μl by gavage. The control groups obtained the corresponding amount of the solvent only, either i.p. or orally. The treatments are performed 5 times on a daily basis. If not indicated otherwise, mice are inspected twice daily for clinical signs (ruffled coat, apathy, hind limb paralysis) until day 21 post infection (p.i.), at which time they are euthanized. |
别名 | 布帕伐醌, Butalex, BW 720C |
分子量 | 326.43 |
分子式 | C21H26O3 |
CAS No. | 88426-33-9 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 3.26 mg/mL (10 mM), Sonification is recommended
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.0634 mL | 15.3172 mL | 30.6344 mL | 76.5861 mL |
5 mM | 0.6127 mL | 3.0634 mL | 6.1269 mL | 15.3172 mL | |
10 mM | 0.3063 mL | 1.5317 mL | 3.0634 mL | 7.6586 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Buparvaquone 88426-33-9 Microbiology/Virology Antibiotic Parasite Inhibitor 布帕伐醌 Butalex BW 720C inhibit inhibitor