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Brassinolide

产品编号 T3151 CAS 72962-43-7

别名: Brassin lactone, 24-Epibrassinolide, 芸苔素内酯

Brassinolide (Brassin lactone) 是一种植物生长调节剂，可调节植物细胞伸长。

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Brassinolide, CAS 72962-43-7

规格	价格/CNY	货期
1 mg	￥ 185	现货
5 mg	￥ 413	现货
10 mg	￥ 663	现货
25 mg	￥ 1,070	现货
50 mg	￥ 1,490	现货
100 mg	￥ 2,230	现货
1 mL * 10 mM (in DMSO)	￥ 438	现货

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其他形式的 Brassinolide:

Methylcobalamin
现货
Epibrassinolide
现货

选择批次

纯度: 99.62%

纯度: 98%

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天然产物信息

生物活性

化学信息

存储 & 溶解度

TCMIP信息

参考文献

相关产品

植物来源	天然产物 > 十字花科 > 芸薹属
结构类型	天然产物 > 类固醇

产品描述	Brassinolide (Brassin lactone) is a plant hormone isolated from rapeseed (Brassica napus) promoting stem elongation and cell division.
体外活性	Brassinolide is a plant sterol isolated from the pollen of rape (Brassica napus L.) initially. Brassinolide can induce a time and concentration-dependent cytotoxicity in PC-3 cells. The mode of cell death appears to be predominately apoptosis, as shown by flow-cytometric analysis, fluorescence and transmission electron microscopes. Caspase-3 activity is obviously increased after Brassinolide treatment. Western blot studies indicate that treatment with Brassinolide triggered a time-dependent decrease in the expression of anti-apoptotic protein Bcl-2, which suggests that Brassinolide can induce cytotoxicity in PC-3 cells by triggering apoptosis. Brassinolide might therefore be a promising candidate for the treatment of prostate cancer[1]. Brassinolide is a plant growth modulator, on multidrug resistance (MDR) of human T lymphoblastoid cell line CCRF-VCR 1000 which is obtained by progressively addition of vincristine (VCR) to sensitive CCRF-CEM cells, and to explore preliminarily the mechanism of reversing action. After treatment of Brassinolide under the concentration of 0.001-10 μg/mL, the resistance of CCRF-VCR is reversed partly with the reversing folds respectively as 4.4-11.6. The intracellular accumulation of rhodamine 123 is significantly reduced in the resistant cells. After treatment of Brassinolide, the accumulation increased, the level of fluorescent dye is situated between resistant cells and sensitive cells. No alteration of the catalytic activity of topoisomerase II is found among three groups. The level of protein expression of p53 in resistant cells is higher than that of sensitive cells. After Brassinolide treatment, the expression of p53 in CCRF-VCR cells restored to the level of sensitive cells. Brassinolide can effectively reverse the resistance of CCRF-VCR cells by inhibiting the effusion of drug transported by P-glucoprotein. To down regulate the abnormal expression of p53 maybe one of the mechanisms of reversing MDR for Brassinolide[2].
细胞实验	Brassinolide is dissolved in DMSO and stored, and then diluted with appropriate medium before use[2]. MTT method is used to detect the resistant factor of resistant cell line and the reversing fold after addition of Brassinolide. The intracellular accumulation of rhodamine 123, a fluorescent dye transported by P-glycoprotein is detected by flow cytometry, the catalytic activity of topoisomerase II is assessed by Sulliven method to find the effect of Brassinolide on resistance. The protein expression of p53 is measured using Western blotting in the sensitive cells and resistant cells to explore the effect of Brassinolide[2].

别名	Brassin lactone, 24-Epibrassinolide, 芸苔素内酯
分子量	480.68
分子式	C28H48O6
CAS No.	72962-43-7

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

DMSO: 60 mg/mL (124.82 mM)

溶液配制表

可选溶剂	浓度体积质量	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.0804 mL	10.4019 mL	20.8039 mL	52.0097 mL
	5 mM	0.4161 mL	2.0804 mL	4.1608 mL	10.4019 mL
	10 mM	0.208 mL	1.0402 mL	2.0804 mL	5.201 mL
	20 mM	0.104 mL	0.5201 mL	1.0402 mL	2.6005 mL
	50 mM	0.0416 mL	0.208 mL	0.4161 mL	1.0402 mL
	100 mM	0.0208 mL	0.104 mL	0.208 mL	0.5201 mL

TCMIP相关数据

中药材来源及性味归经

中药材名称	中药材拉丁名	性	味	归经
欧洲油菜	Brassica napus L.	-	―

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稀释计算器

配液计算器

分子量计算器

质量

浓度

容积

分子量

浓度 (起始)

容积 (起始)

浓度 (终)

容积 (终)

溶液体积

质量

配液浓度

参考文献

1. Wu YD, et al. Brassinolide, a plant sterol from pollen of Brassica napus L., induces apoptosis in human prostate cancer PC-3 cells. Pharmazie. 2007 May;62(5):392-5. 2. Xian LJ, et al. Reversing effect of brassinolide on multidrug resistance of-CCRF-VCR12000 cells and a preliminary investigation on its mechanisms. Yao Xue Xue Bao. 2005 Feb;40(2):117-21.

剂量换算

对于不同动物的给药剂量换算，您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL，一共给药动物10 只，您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法：2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL)，如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法：取 50 μL DMSO 主液，加入 300 μL PEG300，混匀澄清，再加 50 μL Tween 80，混匀澄清，再加 600 μL ddH2O, 混匀澄清。

第一步：请输入动物实验的基本信息

剂量

mg/kg

每只动物体重

给药体积

μL

动物数量

第二步：请输入动物体内配方组成，不同的产品配方组成不同，如有配方需求，可先联系我们提供正确的体内配方。

% DMSO+

% +

% Tween 80+

% ddH₂O

%DMSO+

计算重置

计算结果如下:

工作液浓度: mg/ml;

母液配置方法: mg 药物溶于 μL DMSO (母液浓度为 mg/mL)，如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法：取 μL DMSO 主液，加入 μL PEG300，混匀澄清，再加 μL Tween 80，混匀澄清，再加 μL ddH₂O, 混匀澄清。

备注:

要按顺序从左向右依次添加助溶剂。可配合物理方法，如涡流、超声波或热水浴使之帮助溶解。

技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到，包括如何准备库存溶液，如何存储产品，以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

Brassinolide 72962-43-7 Apoptosis Others Brassin lactone 24-Epibrassinolide Inhibitor inhibit 芸苔素内酯 inhibitor

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Brassinolide

存储

溶解度

溶液配制表

中药材来源及性味归经

计算器

输入分子式，点击计算，可计算出产品的分子量。

参考文献

相关产品

相关化合物库

剂量换算

体内实验配液计算器

技术支持

Keywords