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Bestatin hydrochloride

Bestatin hydrochloride

产品编号 T3529   CAS 65391-42-6
别名: 盐酸乌苯美司, Ubenimex hydrochloride, 苯丁抑制素

Bestatin hydrochloride (Ubenimex hydrochloride) 是CD13(Aminopeptidase N)/APN 和leukotriene A4 hydrolase 抑制剂,可研究癌症。

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Bestatin hydrochloride Chemical Structure
Bestatin hydrochloride, CAS 65391-42-6
规格 价格/CNY 货期 数量
2 mg ¥ 223 现货
5 mg ¥ 397 现货
10 mg ¥ 578 现货
25 mg ¥ 987 现货
50 mg ¥ 1,660 现货
100 mg ¥ 2,690 现货
200 mg ¥ 3,930 现货
500 mg ¥ 6,420 现货
1 mL * 10 mM (in DMSO) ¥ 397 现货
其他形式的 Bestatin hydrochloride:
产品目录号及名称: Bestatin hydrochloride (T3529)
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纯度: 98%
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存储 & 溶解度
参考文献
产品描述 Bestatin hydrochloride (Ubenimex hydrochloride) is an inhibitor of aminopeptidase N (APN)/CD13 and aminopeptidase B.
体外活性 Bestatin, also known as Ubenimex (INN), is a competitive protease inhibitor. It is an inhibitor of aminopeptidase B, leukotriene A4 hydrolase, aminopeptidase N. It is being studied for use in the treatment of acute myelocytic leukemia. Bestatin can be administered, with low toxicity, to cultured cells, intact animals and humans. It has become a useful tool in elucidating the physiological role of some mammalian exopeptidases in the regulation of the immune system, in the growth of tumors and their invasion of surrounding tissues, and in the degradation of cellular proteins.
体内活性 Bestatin is an active anti-angiogenic agent that may inhibit tumor angiogenesis in vivo and tube-like formation of endothelial cells in vitro through its inhibition of APN/CD13 activity. Oral administration of Bestatin (100-200 mg/kg/day) is found to significantly inhibit the melanoma cell-induced angiogenesis in a mouse dorsal air sac assay.
激酶实验 Cells are harvested, washed, and lysed in NP-40 lysis buffer (50 mM Tris-HCl [pH 7.5], 150 mM NaCl, 0.5% NP-40). Total cell protein is quantified using the Bradford assay and 1-mg/mL protein aliquots are made. Ten microliters of total cell protein is mixed with 290 μL of substrate solution (0.1 mg/mL dithiothreitol [DTT], 0.1 mg/mL albumin, and 1 mM alanine-β-naphthylamide). Fluorometric measurements (340 nm excitation, 400 nm emission) are made after 15 and 30 min. The slope of the line between the 15- and 30-min measurements is used to represent aminopeptidase activity. Total cell protein is preincubated with bestatin, amastatin, puromycin, EDTA, and/or ZnCl2 for 20 min before the fluorometric aminopeptidase assay.
细胞实验 Growing cells (1×106 to 2×106 cells/mL) are diluted to 1.0×103 cells/mL and transferred (3 mL) into a well in a 12-well multiwell plate (2.5-cm diameter/well). Cells are treated with 0, 10, 50, 100, 300, or 600 μM Bestatin and allowed to grow at 21°C shaking at 180 rpm for 48 h. A hemocytometer is used to measure cell density after 0, 24, and 48 h.
别名 盐酸乌苯美司, Ubenimex hydrochloride, 苯丁抑制素
分子量 344.84
分子式 C16H25ClN2O4
CAS No. 65391-42-6

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

DMSO: 50 mg/mL (144.99 mM)

H2O: 20 mg/mL (58.00 mM), Sonification is recommended

溶液配制表

可选溶剂 浓度 体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 2.8999 mL 14.4995 mL 28.999 mL 72.4974 mL
5 mM 0.58 mL 2.8999 mL 5.7998 mL 14.4995 mL
10 mM 0.29 mL 1.4499 mL 2.8999 mL 7.2497 mL
20 mM 0.145 mL 0.725 mL 1.4499 mL 3.6249 mL
50 mM 0.058 mL 0.29 mL 0.58 mL 1.4499 mL
DMSO 100 mM 0.029 mL 0.145 mL 0.29 mL 0.725 mL

计算器

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参考文献

1. Hossain A, et al. Protective effects of bestatin in the retina of streptozotocin-induced diabetic mice. Exp Eye Res. 2016 Aug;149:100-6. 2. Qian X, et al. Inhibition of p38 MAPK Phosphorylation Is Critical for Bestatin to Enhance ATRA-Induced Cell Differentiation in Acute Promyelocytic Leukemia NB4 Cells. Am J Ther. 2016 May-Jun;23(3):e680-9. 3. Lis M, et al. The effects of bestatin on humoral response to sheep erythrocytes in non-treated and cyclophosphamide-immunocompromised mice. Immunopharmacol Immunotoxicol. 2013 Feb;35(1):133-8. 4. Poloz Y, et al. Bestatin inhibits cell growth, cell division, and spore cell differentiation in Dictyostelium discoideum. Eukaryot Cell. 2012 Apr;11(4):545-57. 5. Zhu X, Liu S, Wang X, et al. Contribution of porcine aminopeptidase N to porcine deltacoronavirus infection[J]. Emerging microbes & infections. 2018 Apr 11;7(1):65.

文献引用

1. Zhu X, Liu S, Wang X, et al. Contribution of porcine aminopeptidase N to porcine deltacoronavirus infection. Emerging Microbes & Infections. 2018 Apr 11;7(1):65
α-Cyclopentyl-4-(2-quinolinylmethoxy)benzeneacetic acid Methyl arachidate DG051 Betulinic acid L(+)-Leucinol Aminopeptidase-IN-1 ARM1 Acebilustat

相关化合物库

该产品包含在如下化合物库中:
抗癌药物库 微生物天然产物库 抗癌活性化合物库 抗癌上市药物库 抗癌临床化合物库 抑制剂库 高通量筛选天然产物库 临床前化合物库 已知活性化合物库 天然产物库

剂量换算

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体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。

体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。

第一步:请输入动物实验的基本信息
剂量
mg/kg
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g
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μL
动物数量
第二步:请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
% DMSO
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% ddH2O
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技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

Bestatin hydrochloride 65391-42-6 Cytoskeletal Signaling Metabolism Microbiology/Virology Integrin Aminopeptidase Antibacterial Antibiotic Bestatin Hydrochloride Bestatin Inhibitor 盐酸乌苯美司 Ubenimex hydrochloride Bacterial 苯丁抑制素 inhibit Ubenimex Hydrochloride Ubenimex inhibitor

 

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